Shao Wenhua, Ichimura-Shimizu Mayuko, Ogawa Hirohisa, Jin Shengjian, Sutoh Mitsuko, Nakamura Satoko, Onodera Miki, Tawara Hirosuke, Toyohara Shunji, Hokao Ryoji, Kudo Yasusei, Oya Takeshi, Tsuneyama Koichi
Department of Molecular Pathology, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan.
Department of Oral Bioscience, Tokushima University Graduate School of Biomedical Sciences, Tokushima, Japan.
Heliyon. 2023 Jun 3;9(6):e16978. doi: 10.1016/j.heliyon.2023.e16978. eCollection 2023 Jun.
Biopsy is a commonly used method for determining pathological diagnoses by directly using human tissues and cells. Biopsies are widely used to determine disease progression and treatment efficacy. Although organs and tissues are usually obtained by sacrifice during animal experiments, it is theoretically possible to use the same biopsy techniques in humans. In the present study, we examined the feasibility of performing four repeated liver biopsies in a spontaneous metabolic syndrome mouse model. Even though a small number of mice died accidently, most mice were able to undergo four liver biopsies without significant adverse events. We also performed three liver biopsies in mouse liver tumor carcinogen models at 4, 8, and 12 weeks of age. In addition to the sample collected at 16 weeks of age during sacrifice, we successfully collected four liver samples from the same mice at different stages of disease progression. The application of this liver biopsy technique might make it possible for direct evaluation of pathological conditions in the same individual over time, thereby reducing the number of experimental animals.
活检是一种通过直接使用人体组织和细胞来确定病理诊断的常用方法。活检被广泛用于确定疾病进展和治疗效果。尽管在动物实验中通常通过处死来获取器官和组织，但从理论上讲，相同的活检技术也可用于人类。在本研究中，我们检测了在自发性代谢综合征小鼠模型中进行四次重复肝脏活检的可行性。尽管有少数小鼠意外死亡，但大多数小鼠能够接受四次肝脏活检且无明显不良事件。我们还在4周、8周和12周龄的小鼠肝肿瘤致癌物模型中进行了三次肝脏活检。除了在16周龄处死时采集的样本外，我们成功地在疾病进展的不同阶段从同一小鼠身上采集了四个肝脏样本。这种肝脏活检技术的应用可能使随时间直接评估同一个体的病理状况成为可能，从而减少实验动物的数量。
