鉴定超重 2 型糖尿病患者脂肪组织中的 tRNA 衍生 RNA 及其潜在的生物学功能。
Identification of tRNA-derived RNAs in adipose tissue from overweight type 2 diabetes mellitus patients and their potential biological functions.
机构信息
Department of Endocrinology, Hwa Mei Hospital, University of Chinese Academy of Sciences, Ningbo, Zhejiang, China.
出版信息
Front Endocrinol (Lausanne). 2023 Jul 6;14:1139157. doi: 10.3389/fendo.2023.1139157. eCollection 2023.
BACKGROUND
Type 2 diabetes mellitus (T2DM)causes a huge public health burden worldwide, especially for those who are overweight or obese, the pain is often greater. And search for effective targets in overweight T2DM could help improve patient quality of life and prognosis. tRNA-derived RNAs (tsRNAs) are multifunctional regulators that are currently receiving much attention, but there is still a lack of knowledge about tsRNAs in overweight T2DM.
METHODS
T2DM patients with BMI ≥ 25 (Overweight group) and BMI< 25 (Control group) were subjected to tsRNA sequencing; differentially expressed tsRNAs in the two groups were analyzed and their expression was verified using qRT-PCR. The biological function of downstream target genes was also evaluated by enrichment analysis.
RESULTS
qRT-PCR evaluation identified a tsRNA with up-regulated expression (tRF-1-28-Glu-TTC-3-M2) and a tsRNA with down-regulated expression (tRF-1-31-His-GTG-1), both of which may be involved in metabolic and energy-related processes.
CONCLUSION
Dysregulation of tsRNA expression in overweight patients with T2DM suggests a potential role for tsRNA in the development of T2DM.
背景
2 型糖尿病（T2DM）在全球范围内造成了巨大的公共健康负担，尤其是对于超重或肥胖的患者来说，痛苦往往更大。寻找超重 T2DM 中的有效靶点可能有助于改善患者的生活质量和预后。tRNA 衍生的 RNA（tsRNA）是多功能调节剂，目前受到广泛关注，但超重 T2DM 中的 tsRNA 知识仍然缺乏。
方法
对 BMI≥25（超重组）和 BMI<25（对照组）的 T2DM 患者进行 tsRNA 测序；分析两组之间差异表达的 tsRNA，并通过 qRT-PCR 验证其表达。还通过富集分析评估下游靶基因的生物学功能。
结果
qRT-PCR 评价鉴定出一种上调表达的 tsRNA（tRF-1-28-Glu-TTC-3-M2）和一种下调表达的 tsRNA（tRF-1-31-His-GTG-1），它们都可能参与代谢和能量相关过程。
结论
超重 T2DM 患者中 tsRNA 表达的失调表明 tsRNA 在 T2DM 的发展中可能具有潜在作用。
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