Laboratory of Membrane Biology and Biophysics, The Rockefeller University;
J Vis Exp. 2023 Jul 7(197). doi: 10.3791/64769.
Cell culture is a delicate skill necessary for growing human, animal, and insect cells, or other tissues, in a controlled environment. The goal of the protocol is to emphasize the correct techniques used in a research laboratory to prevent contamination from fungi and bacteria. Special emphasis is placed on avoiding mycoplasma contamination, a major concern in the cell culture room due to its small size and resistance to most antibiotics used for cell culture. These same techniques ensure continuous growth and maintain healthy cells. For new and experienced cell culture users alike, it's important to consistently adhere to these best practices to mitigate the risk of contamination. Once a year, laboratories should review cell culture best practices and follow-up with a discussion or additional training if needed. Taking early action to prevent contamination in the first place will save time and money, as compared to cleaning up after contamination occurs. Universal best practices keep cell cultures healthy, thereby reducing the need to constantly thaw new cells, purchase expensive cell culture media, and reducing the amount of incubator decontamination and downtime.
细胞培养是一项精细的技能，需要在受控环境中培养人类、动物和昆虫细胞或其他组织。该方案的目标是强调在研究实验室中使用的正确技术，以防止真菌和细菌污染。特别强调避免支原体污染，由于其体积小且对大多数用于细胞培养的抗生素具有抗性，因此在细胞培养室中这是一个主要关注点。这些相同的技术可确保细胞持续生长并保持健康。对于新的和有经验的细胞培养用户来说，始终如一地遵守这些最佳实践以降低污染风险非常重要。每年，实验室都应审查细胞培养最佳实践，如果需要，可进行讨论或额外培训。与污染发生后再进行清理相比，首先采取措施预防污染将节省时间和金钱。通用最佳实践可保持细胞培养健康，从而减少频繁解冻新细胞、购买昂贵的细胞培养基的需求，并减少培养箱消毒和停机时间。
