van Buul-Offers S, Ueda I, Van den Brande J L
Pediatr Res. 1986 Sep;20(9):825-7. doi: 10.1203/00006450-198609000-00002.
An Escherichia coli derived somatomedin-C/IGF-I preparation (rec-IGF-I) with an amino acid sequence identical to the natural IGF-I derived from human plasma, increases body length and weight, as well as the growth of several organs of Snell dwarf mice, when administered for 4 wk. After 2 wk of treatment rec-IGF-I (22.2 micrograms/day) induced a significant increase over buffer treated controls, to a comparable degree as obtained with bacterially synthesized human growth hormone (bhGH; 8.4 micrograms/day). The weight/length ratio of rec-IGF-I and bhGH-treated dwarf mice after 4 wk of treatment were not significantly different. A significant increase over controls was obtained with both preparations. Organs with increased weights after bhGH treatment (brain; submandibular salivary glands; heart, liver, kidneys, thymus, and spleen) were also heavier after rec-IGF-I. Significance was only reached for the kidneys and the spleen and the musculus quadriceps femoris. Organ weights expressed as a percentage of body weight of bhGH and rec-IGF-I treated dwarfs were similar except for the relative weight of the heart of the bhGH group, which was significantly increased compared to the controls and the rec-IGF-I group. These data resolve the issue as to whether or not pure SM-C/IGF-I will induce growth in length and demonstrate the usefulness of recombinant IGF-I in the studies of growth regulation.
一种氨基酸序列与源自人血浆的天然胰岛素样生长因子-I（IGF-I）相同的大肠杆菌衍生的生长调节素-C/IGF-I制剂（重组IGF-I），在给药4周时，可增加斯内尔侏儒小鼠的体长、体重以及多个器官的生长。治疗2周后，重组IGF-I（22.2微克/天）比缓冲液处理的对照组有显著增加，达到了与细菌合成的人生长激素（bhGH；8.4微克/天）相当的程度。治疗4周后，重组IGF-I和bhGH处理的侏儒小鼠的体重/体长比无显著差异。两种制剂均比对照组有显著增加。bhGH治疗后重量增加的器官（脑；下颌下唾液腺；心脏、肝脏、肾脏、胸腺和脾脏）在重组IGF-I治疗后也更重。仅肾脏、脾脏和股四头肌达到显著差异。除bhGH组心脏的相对重量与对照组和重组IGF-I组相比显著增加外，bhGH和重组IGF-I处理的侏儒小鼠的器官重量占体重的百分比相似。这些数据解决了纯生长调节素-C/IGF-I是否会诱导体长增长的问题，并证明了重组IGF-I在生长调节研究中的有用性。
