van Buul-Offers S, Van den Brande J L
Horm Metab Res. 1985 Jan;17(1):20-4. doi: 10.1055/s-2007-1013437.
Bacterially synthesized human growth hormone (bhGH) administered to Snell dwarf mice during 4 weeks, induced an increase in body length and weight to a comparable degree as obtained with pituitary-derived human growth hormone (hGH). At a dose of 150 mU/day both bhGH and hGH induced a significant stimulation over saline-treated controls, of the weight of the submandibular salivary glands, the m. quadriceps femoris and gastrocnemius, the heart, liver, kidneys, thymus and spleen. The weight of the brain and the thickness of the skinfold were not influenced by either of the preparations used. When organ weights were expressed as a function of body weight, the contribution of the kidneys to body weight was significantly higher with hGH than with bhGH. The other organs studied did not show differences. As a biochemical parameter of cartilage growth, the sulfate incorporation into costal and epiphyseal cartilage in vitro was measured, and it was found to be stimulated by both hormones after short-term treatment. Thus bacterially synthesized hGH behaves identically to pituitary-derived hGH with respect to body length, sulfate incorporation into costal and epiphyseal cartilage, body weight and organ growth of Snell dwarf mice, with one exception: increase of weight of the kidneys, as a function of body weight, was more pronounced after treatment with hGH than with bhGH.
给斯内尔侏儒小鼠连续4周注射细菌合成的人生长激素（bhGH），其体长和体重的增加程度与垂体来源的人生长激素（hGH）相当。每天注射150 mU的bhGH和hGH，均能显著刺激下颌下唾液腺、股四头肌和腓肠肌、心脏、肝脏、肾脏、胸腺和脾脏的重量，使其高于生理盐水处理的对照组。所用的两种制剂均未影响脑重量和皮褶厚度。当器官重量以体重的函数表示时，hGH组肾脏占体重的比例显著高于bhGH组。其他研究的器官未显示出差异。作为软骨生长的生化参数，测量了体外肋软骨和骨骺软骨中硫酸盐的掺入量，发现短期治疗后两种激素均能刺激其掺入。因此，就斯内尔侏儒小鼠的体长、肋软骨和骨骺软骨中硫酸盐的掺入、体重和器官生长而言，细菌合成的hGH与垂体来源的hGH表现相同，但有一个例外：与bhGH相比，hGH治疗后肾脏重量随体重增加更为明显。
