Sánchez Rivera Francisco J, Dow Lukas E
David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA
Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA.
Cold Spring Harb Perspect Med. 2024 May 2;14(5):a041384. doi: 10.1101/cshperspect.a041384.
Cancers arise through acquisition of mutations in genes that regulate core biological processes like cell proliferation and cell death. Decades of cancer research have led to the identification of genes and mutations causally involved in disease development and evolution, yet defining their precise function across different cancer types and how they influence therapy responses has been challenging. Mouse models have helped define the in vivo function of cancer-associated alterations, and genome-editing approaches using CRISPR have dramatically accelerated the pace at which these models are developed and studied. Here, we highlight how CRISPR technologies have impacted the development and use of mouse models for cancer research and discuss the many ways in which these rapidly evolving platforms will continue to transform our understanding of this disease.
癌症是通过获得调控细胞增殖和细胞死亡等核心生物学过程的基因突变而产生的。数十年的癌症研究已导致鉴定出因果参与疾病发生和演变的基因和突变，然而，确定它们在不同癌症类型中的精确功能以及它们如何影响治疗反应一直具有挑战性。小鼠模型有助于确定癌症相关改变的体内功能，并且使用CRISPR的基因组编辑方法极大地加快了这些模型的开发和研究速度。在这里，我们强调CRISPR技术如何影响用于癌症研究的小鼠模型的开发和使用，并讨论这些快速发展的平台将继续改变我们对这种疾病理解的多种方式。
