Forestier F, Boneu B, Potron G, Boisseau M, Coqueret B, Saffar S, Chambrette B
Nouv Rev Fr Hematol (1978). 1986;28(3):157-62.
The aim of this work was to study certain causes of variation in the results of laboratory monitoring of treatment with vitamin K antagonists. Four centers participated in the study. In the initial phase, each center performed fifteen measurements of prothrombin time (PT) and activated partial thromboplastin time (APTT) on the same standard lyophilized plasma using its own usual reagents and its own methodology (protocol I). In the second phase of this study, each laboratory performed PT and APTT measurements on 30 frozen plasma specimens from patients receiving long term treatment with vitamin K antagonists using protocol I and protocol II (common reagents but own methodology). In the third phase, plasma from 19 patients receiving long term therapy with vitamin K antagonists were tested with common reagents and a standardized methodology (protocol III). the intralaboratory reproductability was very good; however, the use of common reagents and the standardization of methods greatly improved the intercenter reproductability. The use of common reagents allowed a stricter and a less contradictory interpretation of the tests.
这项工作的目的是研究维生素K拮抗剂治疗实验室监测结果变化的某些原因。四个中心参与了该研究。在初始阶段，每个中心使用自己常用的试剂和方法（方案I）对同一标准冻干血浆进行15次凝血酶原时间（PT）和活化部分凝血活酶时间（APTT）测量。在本研究的第二阶段，每个实验室使用方案I和方案II（通用试剂但各自方法）对30份接受维生素K拮抗剂长期治疗患者的冷冻血浆标本进行PT和APTT测量。在第三阶段，使用通用试剂和标准化方法（方案III）对19名接受维生素K拮抗剂长期治疗患者的血浆进行检测。实验室内的可重复性非常好；然而，使用通用试剂和方法标准化极大地提高了中心间的可重复性。通用试剂的使用使得对检测结果的解释更严格且矛盾更少。
