犬、山羊和豚鼠血管活性肠肽的纯化及氨基酸序列

Purification and amino acid sequences of dog, goat and guinea pig VIPs.

作者信息

Eng J, Du B H, Raufman J P, Yalow R S

出版信息

Peptides. 1986;7 Suppl 1:17-20. doi: 10.1016/0196-9781(86)90158-0.

Abstract

VIP has been reported to be identical in pig, cow, human and rat but to differ in four amino acids in chicken. We report now on the purification and sequences of VIP from three other mammalian species, dog, goat and guinea pig (GP). The general method of purification of the three peptides was similar. The frozen intestines were extracted in five volumes of an organic solvent and the residual cakes re-extracted with acid or acid-ethanol. The VIPs were brought to final purity through a series of HPLC steps. Overall recovery using this methodology is in the range of 20-30%. The VIP sequences obtained were: Dog and Goat: (sequence in text) Dog and goat VIP are identical to, but GP VIP differs from, that of other mammalian species. Substitution of the four amino acids in positions 5, 9, 19 and 26 appears not to affect its biological activity. That GP VIP differs from other mammalian VIP's is further evidence that the GP gastroenteropancreatic axis has a unique evolutionary separation from other mammals.

摘要

据报道，血管活性肠肽（VIP）在猪、牛、人类和大鼠中是相同的，但在鸡中有四个氨基酸不同。我们现在报告从其他三种哺乳动物，即狗、山羊和豚鼠（GP）中纯化和测序VIP的情况。这三种肽的纯化方法大致相似。将冷冻的肠道用五倍体积的有机溶剂提取，剩余的饼状物再用酸或酸 - 乙醇提取。通过一系列高效液相色谱步骤使VIP达到最终纯度。使用这种方法的总体回收率在20% - 30%范围内。获得的VIP序列如下：狗和山羊：（文本中的序列）狗和山羊的VIP与其他哺乳动物的相同，但豚鼠的VIP与之不同。第5、9、19和26位的四个氨基酸的替换似乎不影响其生物活性。豚鼠的VIP与其他哺乳动物的VIP不同，这进一步证明豚鼠的胃肠胰轴与其他哺乳动物有独特的进化分离。