Khubiev Omar M, Egorov Anton R, Lobanov Nikolai N, Fortalnova Elena A, Kirichuk Anatoly A, Tskhovrebov Alexander G, Kritchenkov Andreii S
Faculty of Science, Peoples' Friendship University of Russia (RUDN University), Miklukho-Maklaya St. 6, 117198 Moscow, Russia.
Institute of Technical Acoustics NAS of Belarus, Ludnikova Prosp. 13, 210009 Vitebsk, Belarus.
BioTech (Basel). 2023 Jul 7;12(3):50. doi: 10.3390/biotech12030050.
In this study, we elaborated new chitosan-based films reinforced by iron(III)-containing chitosan nanoparticles Fe(III)-CS-NPs at different concentrations. We found that the optimum concentration of Fe(III)-CS-NPs for the improvement of antibacterial and mechanical properties of the films was 10% (σ = ca. 8.8 N/mm, ε = ca. 41%, inhibition zone for = ca. 16.8 mm and for = ca. 11.2 mm). Also, using the click-chemistry approach (thiol-ene reaction), we have synthesized a novel water-soluble cationic derivative of chitin. The addition of this derivative of chitin to the chitosan polymer matrix of the elaborated film significantly improved its mechanical (σ = ca. 11.6 N/mm, ε = ca. 75%) and antimicrobial (inhibition zone for = ca. 19.6 mm and for = ca. 14.2 mm) properties. The key mechanism of the antibacterial action of the obtained films is the disruption of the membranes of bacterial cells. The elaborated antibacterial films are of interest for potential biomedical and food applications.
在本研究中，我们制备了以不同浓度含三价铁壳聚糖纳米粒子（Fe(III)-CS-NPs）增强的新型壳聚糖基薄膜。我们发现，用于改善薄膜抗菌性能和机械性能的Fe(III)-CS-NPs的最佳浓度为10%（σ约为8.8 N/mm，ε约为41%，对大肠杆菌的抑菌圈约为16.8 mm，对金黄色葡萄球菌的抑菌圈约为11.2 mm）。此外，我们采用点击化学方法（硫醇-烯反应）合成了一种新型的水溶性几丁质阳离子衍生物。将这种几丁质衍生物添加到所制备薄膜的壳聚糖聚合物基质中，显著改善了其机械性能（σ约为11.6 N/mm，ε约为75%）和抗菌性能（对大肠杆菌的抑菌圈约为19.6 mm，对金黄色葡萄球菌的抑菌圈约为14.2 mm）。所得薄膜抗菌作用的关键机制是破坏细菌细胞膜。所制备的抗菌薄膜在潜在的生物医学和食品应用方面具有吸引力。
