Department of Neurology, Wan Fang Hospital, Taipei Medical University, Taipei, Taiwan.
The Ph.D. Program for Translational Medicine, Taipei Medical University and Academia Sinica, New Taipei City, Taiwan.
Exp Physiol. 2024 Jan;109(1):66-80. doi: 10.1113/EP090762. Epub 2023 Jul 25.
Although acid-sensing ion channels (ASICs) are proton-gated ion channels responsible for sensing tissue acidosis, accumulating evidence has shown that ASICs are also involved in neurosensory mechanotransduction. However, in contrast to Piezo ion channels, evidence of ASICs as mechanically gated ion channels has not been found using conventional mechanoclamp approaches. Instead, ASICs are involved in the tether model of mechanotransduction, with the channels gated via tethering elements of extracellular matrix and intracellular cytoskeletons. Methods using substrate deformation-driven neurite stretch and micropipette-guided ultrasound were developed to reveal the roles of ASIC3 and ASIC1a, respectively. Here we summarize the evidence supporting the roles of ASICs in neurosensory mechanotransduction in knockout mouse models of ASIC subtypes and provide insight to further probe their roles in proprioception.
尽管酸敏离子通道（ASICs）是感应组织酸中毒的质子门控离子通道，但越来越多的证据表明，ASICs 也参与神经感觉机械转导。然而，与 Piezo 离子通道不同，使用传统的机械钳方法并未发现 ASICs 作为机械门控离子通道的证据。相反，ASICs 参与机械转导的拴系模型，通道通过细胞外基质和细胞内细胞骨架的拴系元件门控。使用基于底物变形的神经突拉伸和微管引导超声的方法分别开发出来，以揭示 ASIC3 和 ASIC1a 的作用。在这里，我们总结了支持 ASIC 亚型敲除小鼠模型中 ASIC 在神经感觉机械转导中的作用的证据，并提供了进一步探究其在本体感觉中的作用的见解。
