Lin Shing-Hong, Sun Wei-Hsin, Chen Chih-Cheng
Institute of Life Sciences, National Defense Medical Center, Taipei 114, Taiwan; Institute of Biomedical Sciences, Academia Sinica, Taipei 115, Taiwan.
Department of Life Sciences, National Central University, Jhongli 32054, Taiwan.
Neuropharmacology. 2015 Jul;94:99-118. doi: 10.1016/j.neuropharm.2014.12.011. Epub 2015 Jan 9.
Advanced gene targeting technology and related tools in mice have been incorporated into studies of acid-sensing ion channels (ASICs). A single ASIC subtype can be knocked out specifically and screened thoroughly for expression in the nervous system at the cellular level. Mapping studies have further shed light on the initiation and identification of related behavioral phenotypes. Here we review studies involving genetically engineered mouse models used to investigate the physiological function of individual ASIC subtypes: ASIC1 (and ASIC1a), ASIC2, ASIC3 and ASIC4. We discuss the detailed expression studies and significant phenotypes revealed with gene knockout for most known Asic subtypes. Each strategy designed to manipulate mouse genetics has advantages and disadvantages. We discuss the limitations of these Asic-knockout models and propose future directions to solve the genetic issues. This article is part of the Special Issue entitled 'Acid-Sensing Ion Channels in the Nervous System'.
先进的小鼠基因靶向技术及相关工具已被应用于酸敏感离子通道（ASICs）的研究中。单一的ASIC亚型能够被特异性敲除，并在细胞水平上对其在神经系统中的表达进行全面筛选。图谱研究进一步揭示了相关行为表型的起始和识别。在此，我们综述了涉及用于研究单个ASIC亚型（ASIC1（及ASIC1a）、ASIC2、ASIC3和ASIC4）生理功能的基因工程小鼠模型的研究。我们讨论了针对大多数已知Asic亚型通过基因敲除所揭示的详细表达研究和重要表型。每种旨在操控小鼠遗传学的策略都有其优缺点。我们讨论了这些Asic敲除模型的局限性，并提出了解决遗传问题的未来方向。本文是名为“神经系统中的酸敏感离子通道”特刊的一部分。
