College of Ethnic Medicine, Yunnan University of Chinese Medicine, Yunnan, Kunming, 650500, People's Republic of China.
College of Ethnic Medicine, Yunnan University of Chinese Medicine, Yunnan, Kunming, 650500, People's Republic of China; Yunnan Key Laboratory of Dai and Yi Medicines, Yunnan University of Chinese Medicine, Kunming, 650500, Yunnan, People's Republic of China.
J Ethnopharmacol. 2024 Jan 10;318(Pt A):116921. doi: 10.1016/j.jep.2023.116921. Epub 2023 Jul 23.
An essential factor related to the acute alcoholic liver injury is damage to the intestinal mucosal barrier. Yajieshaba (YJSB) is a commonly used formulation of Dai people in China and protects the liver.
This study investigated whether YJSB can prevent acute alcoholic liver injury by regulating the intestinal mucosal barrier.
The mice received 0.39 g/kg, 1.17 g/kg, and 3.51 g/kg dose YJSB for 7 days, a mouse model of acute alcoholic liver injury was established by a single instillation of 56% alcohol. Plasma biochemical markers were analyzed, liver injury was identified by histopathology, lipopolysaccharide (LPS), nuclear factor-k-gene binding (NF-κB), hepatic inflammatory factors, oxidative stress factors and reactive oxygen species (ROS) content was analyzed. The morphological changes of intestinal histology were observed by H&E staining, and the ultrastructure of ileal cells was observed by transmission electron microscopy. Immunofluorescence and Western blot was used to determine the expression levels of transporters and enzymes involved in Claudin 1, Occludin and zona occludens 1 (ZO-1) homeostasis in the liver and intestine.
The findings showed that YJSB reduced the levels of aspartate aminotr ansferase (AST), alanine aminotransferase (ALT) and total bile acid (TBA), both of which are indicators of liver function and had a protective effect against liver injury. In the liver homogenate, YJSB reduced the level of LPS, NF-κB, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6), decreased the level of superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GSH-PX) and catalase (CAT) and ROS. The results of hematoxylin and eosin (H&E) staining and transmission electron microscopy analysis revealed that YJSB reduced the degree of damage to intestinal tissue and intracellular organelles, implying that YJSB can reduce the "attack factor" that causes intestinal barrier damage, increase the "defense factor" that protects the intestinal barrier. The results of immunohistochemistry and Western blotting analysis showed that YJSB could increase the expression of claudin 1, occludin, and ZO-1 proteins, suggesting that the mechanism of action of YJSB against acute alcohol liver injury involves the upregulation of the expression of the intestinal barrier-related proteins and the repair of the damaged intestinal barrier.
YJSB can block LPS, oxidative stress factors, and other harmful substances in the blood and protect the liver resisting acute alcoholic liver injury.
急性酒精性肝损伤的一个重要因素是肠道黏膜屏障的损伤。雅叫哈什（YJSB）是中国傣族常用的配方，具有保肝作用。
本研究旨在探讨 YJSB 是否通过调节肠道黏膜屏障来预防急性酒精性肝损伤。
将小鼠给予 0.39g/kg、1.17g/kg 和 3.51g/kg 的 YJSB 剂量，连续 7 天，通过单次灌注 56%酒精建立急性酒精性肝损伤模型。分析血浆生化标志物，通过组织病理学鉴定肝损伤，分析脂多糖（LPS）、核因子-k-基因结合（NF-κB）、肝内炎症因子、氧化应激因子和活性氧（ROS）含量。通过 H&E 染色观察肠组织学形态变化，通过透射电镜观察回肠细胞的超微结构。免疫荧光和 Western blot 用于测定肝脏和肠道中 Claudin 1、Occludin 和 ZO-1 稳态相关转运体和酶的表达水平。
研究结果表明，YJSB 降低了天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）和总胆汁酸（TBA）的水平，这些都是肝功能的指标，对肝损伤具有保护作用。在肝匀浆中，YJSB 降低了 LPS、NF-κB、肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和白细胞介素-6（IL-6）的水平，降低了超氧化物歧化酶（SOD）、丙二醛（MDA）、谷胱甘肽过氧化物酶（GSH-PX）和过氧化氢酶（CAT）和 ROS 的水平。苏木精和伊红（H&E）染色和透射电镜分析的结果表明，YJSB 降低了肠组织和细胞内细胞器的损伤程度，这意味着 YJSB 可以减少导致肠屏障损伤的“攻击因素”，增加保护肠屏障的“防御因素”。免疫组化和 Western blot 分析的结果表明，YJSB 可以增加 Claudin 1、Occludin 和 ZO-1 蛋白的表达，提示 YJSB 防治急性酒精性肝损伤的作用机制涉及上调肠屏障相关蛋白的表达和修复受损的肠屏障。
YJSB 可以阻断血液中的 LPS、氧化应激因子等有害物质，保护肝脏免受急性酒精性肝损伤。
