鸦胆子苦醇通过抑制 NLRP3 炎症小体抑制 LPS 诱导的肠上皮细胞损伤
Yajieshaba prevents lipopolysaccharide-induced intestinal barrier injuryanti-inflammatory and anti-apoptosis.
机构信息
Yunnan Key Laboratory of Dai and Yi Medicines, Yunnan University of Chinese Medicine, Kunming 650500, China.
出版信息
J Tradit Chin Med. 2022 Oct;42(5):707-714. doi: 10.19852/j.cnki.jtcm.2022.05.005.
OBJECTIVE
To investigate the protective effect of Yajieshaba (YJSB) on the intestinal barrier dysfunction induced by lipopolysaccharide (LPS).
METHODS
C57BL/6 mice and rat intestinal epithelial cells were treated with LPS. Thiazolyl Blue Tetrazolium Bromide assay were used to detect cell viability. D-Lactate, diamine oxidase and myeloperoxidase and cytokines were determined by enzyme-linked immunosorbent assay. Western blot was used to detect apoptosis-related proteins and tight junction (TJ) proteins. Real-time quantitative polymerase chain reaction was used to quantify the levels of mRNA expression of cytokines. Histological analysis was performed by hematoxylin and eosin staining. An immunofluorescence staining assay was performed to determine the expression level of TJ protein.
RESULTS
YJSB increased cell viability and decreased apoptosis, maintained intestinal permeability after LPS-induced. YJSB inhibited LPS-induced decrease of TJ protein expression, pro-inflammatory cytokine levels and neutrophil infiltration.
CONCLUSION
YJSB protect against LPS-induced intestinal barrier dysfunction anti-inflammatory and anti-apoptosis, suggesting its therapeutic potential against intestinal barrier injury-related diseases.
目的
研究鸦胆子苦醇（YJSB）对脂多糖（LPS）诱导的肠道屏障功能障碍的保护作用。
方法
用 LPS 处理 C57BL/6 小鼠和大鼠肠上皮细胞。噻唑蓝比色法检测细胞活力。酶联免疫吸附试验测定 D-乳酸、二胺氧化酶和髓过氧化物酶及细胞因子。Western blot 检测凋亡相关蛋白和紧密连接（TJ）蛋白。实时定量聚合酶链反应定量细胞因子 mRNA 表达水平。苏木精和伊红染色进行组织学分析。免疫荧光染色法测定 TJ 蛋白表达水平。
结果
YJSB 增加细胞活力，降低 LPS 诱导后的细胞凋亡，维持肠道通透性。YJSB 抑制 LPS 诱导的 TJ 蛋白表达、促炎细胞因子水平和中性粒细胞浸润降低。
结论
YJSB 对 LPS 诱导的肠道屏障功能障碍具有抗炎和抗凋亡作用，提示其对肠道屏障损伤相关疾病具有治疗潜力。
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