State and Local Joint Engineering Research Centre of Urban Wastewater Treatment and Reclamation, Qingdao University of Technology, Qingdao 266520, PR China.
School of Environmental Science and Engineering, Qingdao University, Qingdao 266071, PR China.
Bioresour Technol. 2023 Nov;387:129550. doi: 10.1016/j.biortech.2023.129550. Epub 2023 Jul 24.
The feasibility of treating wastewater and excess sludge via simultaneous nitritation, anammox, denitrification and fermentation (SNADF) was investigated in three parallel sequencing batch reactors (SBRs). SBR2 and SBR3 received exogenous nitrification-denitrification sludge and thermal hydrolysis sludge, respectively. Nitrogen removal efficiencies of 92.8 ± 5.9%, 94.6 ± 4.1%, 93.4 ± 4.8% were achieved in SBR1, SBR2, and SBR3, respectively (influent ammonium: 56.0-74.0 mg N/L), with low observed sludge yield of 0.02-0.15, -0.06-0.11, -0.17-0.05 kg mixed liquor suspended solids (MLSS)/kg chemical oxygen demand (COD). Anammox bacterial abundances increased from 3.6 × 10 ± 2.8 × 10 to 8.1 × 10 ± 2.3 × 10, 1.5 × 10 ± 1.1 × 10, and 1.4 × 10 ± 2.9 × 10 copies/L in SBR1-SBR3, respectively. The abundances of Nitrosomonas, genes (amo, hao) for partial nitrification, and narGHI genes (nitrate → nitrite) in dominant partial denitrifying bacteria (Candidatus Competibacter) were higher in SBR2 and SBR3 than that in SBR1. These results suggested that adding excess sludge promoted sludge reduction, nitrite production and anammox bacterial enrichment. The SNADF system could treat excess sludge, meanwhile, achieve advanced nitrogen removal.
在三个平行的序批式反应器（SBR）中研究了通过同步硝化反硝化、厌氧氨氧化、反硝化和发酵（SNADF）处理废水和剩余污泥的可行性。SBR2 和 SBR3 分别接收了外加的硝化-反硝化污泥和热水解污泥。SBR1、SBR2 和 SBR3 中的氮去除效率分别为 92.8±5.9%、94.6±4.1%和 93.4±4.8%（进水氨氮：56.0-74.0mg N/L），观察到的污泥产量低至 0.02-0.15、-0.06-0.11 和-0.17-0.05kg 混合液悬浮固体（MLSS）/kg 化学需氧量（COD）。厌氧氨氧化菌的丰度从 3.6×10±2.8×10 增加到 8.1×10±2.3×10、1.5×10±1.1×10 和 1.4×10±2.9×10 拷贝/L，分别在 SBR1-SBR3 中。在 SBR2 和 SBR3 中，亚硝化菌、部分硝化基因（amo、hao）和主导的部分反硝化菌（Candidatus Competibacter）中的 narGHI 基因（硝酸盐→亚硝酸盐）的丰度高于 SBR1。这些结果表明，添加剩余污泥促进了污泥减少、亚硝酸盐生成和厌氧氨氧化菌的富集。SNADF 系统可以处理剩余污泥，同时实现高级氮去除。
