Mendoza-Martínez Ariann E, Sánchez Olivia, Aguirre Jesús
Departamento de Biología Celular y del Desarrollo, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Apartado Postal 70-242, 04510, México, D.F., Mexico.
Departamento de Biología Celular y del Desarrollo, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Apartado Postal 70-242, 04510, México, D.F., Mexico.
Fungal Biol. 2023 Jul-Aug;127(7-8):1198-1208. doi: 10.1016/j.funbio.2022.12.001. Epub 2022 Dec 5.
In addition to their role in the breakdown of HO, some peroxiredoxins (Prxs) have chaperone and HO sensing functions. Acting as an HO sensor, Prx Gpx3 transfers the oxidant signal to the transcription factor Yap1, involved in the antioxidant response in Saccharomyces cerevisiae. We have shown that Aspergillus nidulans Yap1 ortholog NapA is necessary for the antioxidant response, the utilization of arabinose, fructose and ethanol, and for proper development. To address the Prx roles in these processes, we generated and characterized mutants lacking peroxiredoxins PrxA, PrxB, PrxC, or TpxC. Our results show that the elimination of peroxiredoxins PrxC or TpxC does not produce any distinguishable phenotype. In contrast, the elimination of atypical 2-cysteine peroxiredoxins PrxA and PrxB produce different mutant phenotypes. ΔprxA, ΔnapA and ΔprxA ΔnapA mutants are equally sensitive to HO and menadione, while PrxB is dispensable for this. However, the sensitivity of ΔprxA and ΔprxA ΔnapA mutants is increased by the lack of PrxB. Moreover, PrxB is required for arabinose and ethanol utilization and fruiting body cell wall pigmentation. PrxA expression is partially independent of NapA, and the replacement of peroxidatic cysteine 61 by serine (C61S) is enough to cause oxidative stress sensitivity and prevent NapA nuclear accumulation in response to HO, indicating its critical role in HO sensing. Our results show that despite their high similarity, PrxA and PrxB play differential roles in Aspergillus nidulans antioxidant response, carbon utilization and development.
除了在过氧化氢（HO）分解中发挥作用外，一些过氧化物酶（Prxs）还具有伴侣蛋白和HO传感功能。作为HO传感器，Prx Gpx3将氧化信号传递给转录因子Yap1，Yap1参与酿酒酵母的抗氧化反应。我们已经表明，构巢曲霉Yap1的直系同源物NapA对于抗氧化反应、阿拉伯糖、果糖和乙醇的利用以及正常发育是必需的。为了研究Prx在这些过程中的作用，我们构建并鉴定了缺乏过氧化物酶PrxA、PrxB、PrxC或TpxC的突变体。我们的结果表明，消除过氧化物酶PrxC或TpxC不会产生任何可区分的表型。相比之下，消除非典型的2-半胱氨酸过氧化物酶PrxA和PrxB会产生不同的突变表型。ΔprxA、ΔnapA和ΔprxA ΔnapA突变体对HO和甲萘醌同样敏感，而PrxB对此是可有可无的。然而，缺乏PrxB会增加ΔprxA和ΔprxA ΔnapA突变体的敏感性。此外，PrxB是阿拉伯糖和乙醇利用以及子实体细胞壁色素沉着所必需的。PrxA的表达部分独立于NapA，并且将过氧化物半胱氨酸61替换为丝氨酸（C61S）足以导致氧化应激敏感性，并阻止NapA在HO响应时的核积累，表明其在HO传感中起关键作用。我们的结果表明，尽管PrxA和PrxB高度相似，但它们在构巢曲霉的抗氧化反应、碳利用和发育中发挥着不同的作用。
