Suppr超能文献

酿酒酵母的双链和单链RNA病毒。

Double-stranded and single-stranded RNA viruses of Saccharomyces cerevisiae.

作者信息

Wickner R B

机构信息

Section on Genetics of Simple Eukaryotes, National Institute of Diabetes, Digestive and Kidney Diseases, Bethesda, Maryland 20892.

出版信息

Annu Rev Microbiol. 1992;46:347-75. doi: 10.1146/annurev.mi.46.100192.002023.

Abstract

Yeast RNA viruses include L-A (and its toxin-encoding satellites M1, M2, ...) and L-BC dsRNA viruses and the single-stranded replicons 20S RNA and 23S RNA. L-A has a single-segment 4.6-kb linear genome encoding a major coat protein (gag) and its RNA-dependent RNA polymerase (pol), the latter expressed as a gag-pol fusion protein formed by a -1 ribosomal frameshift. In vitro replication, transcription, and binding systems for L-A have been used to define cis sites necessary for packaging and replication of viral RNA. Cellular functions that promote viral replication include the MAK3-encoded N-acetyltransferase whose modification of the gag N terminus is necessary for L-A virus assembly. The toxins encoded by the M satellite RNAs are processed by enzymes (KEX1 and KEX2, for killer expression) whose study led to discovery of mammalian hormone-processing enzymes. 20S RNA is an apparently naked circular RNA replicon (with a dsRNA form called W) encoding a RNA polymerase-like molecule. Its copy number is induced 10,000-fold in 1% potassium acetate, and it is subject to the same SKI antiviral system that represses L-A, L-BC, and M dsRNA copy number.

摘要

酵母RNA病毒包括L-A(及其编码毒素的卫星病毒M1、M2等)和L-BC双链RNA病毒以及单链复制子20S RNA和23S RNA。L-A有一个单片段4.6 kb的线性基因组,编码一种主要衣壳蛋白(gag)及其RNA依赖性RNA聚合酶(pol),后者通过-1核糖体移码表达为gag-pol融合蛋白。已利用L-A的体外复制、转录和结合系统来确定病毒RNA包装和复制所需的顺式作用位点。促进病毒复制的细胞功能包括由MAK3编码的N-乙酰转移酶,其对gag N端的修饰是L-A病毒组装所必需的。M卫星RNA编码的毒素由酶(用于杀伤表达的KEX1和KEX2)加工,对这些酶的研究导致了哺乳动物激素加工酶的发现。20S RNA是一种明显裸露的环状RNA复制子(具有一种称为W的双链RNA形式),编码一种类似RNA聚合酶的分子。其拷贝数在1%醋酸钾中可诱导增加10000倍,并且它受到与抑制L-A、L-BC和M双链RNA拷贝数相同的SKI抗病毒系统的作用。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验