半乳糖凝集素-9 对人十二指肠腺癌细胞增殖和肿瘤生长的抗肿瘤作用。

Antitumor Effect of Galectin-9 on Cell Proliferation and Tumor Growth of Human Duodenal Adenocarcinoma Cells.

机构信息

Department of Gastroenterology and Neurology, Faculty of Medicine, Kagawa University, Miki-cho, Japan;

Department of Gastroenterology and Neurology, Faculty of Medicine, Kagawa University, Miki-cho, Japan.

出版信息

Anticancer Res. 2023 Aug;43(8):3769-3777. doi: 10.21873/anticanres.16562.

DOI:10.21873/anticanres.16562

PMID:37500141

Abstract

BACKGROUND/AIM: Galectin-9 (Gal-9) induces tumor cell apoptosis in lymphoma and other malignant cell types. Duodenal adenocarcinoma is a rare malignancy, and there are insufficient data to determine a standard therapeutic approach. Here, we investigated the antitumor effect of Gal-9 in HuTu-80 duodenal adenocarcinoma cells.

MATERIALS AND METHODS

Cell proliferation was examined in HuTu-80 cells using a Cell Counting Kit-8 assay. Cell cycle analysis, apoptosis array, and microRNA expression analysis were performed to identify the effect of Gal-9 on HuTu-80 cells. The antitumor effect of Gal-9 was also examined using xenograft mouse models.

RESULTS

Gal-9 suppressed the proliferation of HuTu-80 via blockade of the G to G cell cycle transition. This blockade was accompanied by a strong decrease in cyclin D1 and phosphorylated Rb, suggesting a G arrest. Additionally, Gal-9 induced apoptosis, and the expression of cleaved caspase-3 was increased in Gal-9-treated HuTu-80 cells according to the apoptosis array. MiRNA microarrays revealed that Gal-9 altered the expression of miRNAs in HuTu-80 cells.

CONCLUSION

These data demonstrate the therapeutic potential of Gal-9 and provide molecular mechanistic insights into its antitumor effect in HuTu-80 cells.

摘要

背景/目的：半乳糖凝集素-9（Gal-9）可诱导淋巴瘤和其他恶性细胞类型的肿瘤细胞凋亡。十二指肠腺癌是一种罕见的恶性肿瘤，目前尚无足够的数据来确定标准的治疗方法。在这里，我们研究了 Gal-9 在 HuTu-80 十二指肠腺癌细胞中的抗肿瘤作用。

材料和方法

使用 Cell Counting Kit-8 检测试剂盒检测 HuTu-80 细胞中的细胞增殖。进行细胞周期分析、凋亡分析和 microRNA 表达分析，以确定 Gal-9 对 HuTu-80 细胞的作用。还使用异种移植小鼠模型检查了 Gal-9 的抗肿瘤作用。

结果

Gal-9 通过阻断 G 期到 G 期的细胞周期转换抑制 HuTu-80 的增殖。这种阻断伴随着细胞周期蛋白 D1 和磷酸化 Rb 的强烈减少，表明 G 期阻滞。此外，Gal-9 诱导细胞凋亡，并且根据凋亡分析，Gal-9 处理的 HuTu-80 细胞中 cleaved caspase-3 的表达增加。miRNA 微阵列显示 Gal-9 改变了 HuTu-80 细胞中 miRNAs 的表达。