Takano Jitsuko, Morishita Asahiro, Fujihara Shintaro, Iwama Hisakazu, Kokado Fuyuko, Fujikawa Keiko, Fujita Koji, Chiyo Taiga, Tadokoro Tomoko, Sakamoto Teppei, Nomura Takako, Tani Joji, Miyoshi Hisaaki, Yoneyama Hirohito, Kobara Hideki, Mori Hirohito, Niki Toshihiro, Hirashima Mitsuomi, Masaki Tsutomu
Department of Gastroenterology and Neurology, Kagawa University School of Medicine, Kagawa 761-0793, Japan.
Life Science Research Center, Kagawa University School of Medicine, Kagawa 761-0793, Japan.
Oncol Rep. 2016 Feb;35(2):851-60. doi: 10.3892/or.2015.4452. Epub 2015 Nov 26.
Gastric cancer is the second-leading cause of cancer-related mortality worldwide, and the prognosis of advanced gastric cancer remains poor. Galectin-9 (Gal-9) is a tandem-repeat-type galectin that has recently been demonstrated to exert anti-proliferative effects on various types of cancer cells. The aim of our present study was to evaluate the effects of Gal-9 on human gastric cancer cells and the expression levels of microRNAs (miRNAs) associated with the antitumor effects of Gal-9 in vitro. In our initial experiments, Gal-9 suppressed the proliferation of gastric cancer cell lines in vitro. Our data further revealed that Gal-9 increased caspase-cleaved keratin 18 (CCK18) levels in gastric cancer cells. Additionally, Gal-9 reduced the phosphorylation of vascular endothelial growth factor receptor-3 (VEGFR-3) and insulin-like growth factor-1 receptor (IGF-1R). Furthermore, miRNA expression levels were markedly altered with Gal-9 treatment in vitro. In conclusion, Gal-9 suppressed the proliferation of human gastric cancer cells by inducing apoptosis. These findings suggest that Gal-9 could be a potential therapeutic target in the treatment of gastric cancer.
胃癌是全球癌症相关死亡的第二大原因，晚期胃癌的预后仍然很差。半乳糖凝集素-9（Gal-9）是一种串联重复型半乳糖凝集素，最近已被证明对各种类型的癌细胞具有抗增殖作用。我们目前研究的目的是评估Gal-9对人胃癌细胞的影响以及体外与Gal-9抗肿瘤作用相关的微小RNA（miRNA）的表达水平。在我们最初的实验中，Gal-9在体外抑制了胃癌细胞系的增殖。我们的数据进一步显示，Gal-9增加了胃癌细胞中半胱天冬酶切割角蛋白18（CCK18）的水平。此外，Gal-9降低了血管内皮生长因子受体-3（VEGFR-3）和胰岛素样生长因子-1受体（IGF-1R）的磷酸化。此外，体外Gal-9处理后miRNA表达水平发生了明显改变。总之，Gal-9通过诱导凋亡抑制人胃癌细胞的增殖。这些发现表明，Gal-9可能是治疗胃癌的潜在治疗靶点。
