Bioprocess Laboratory, Chemical Engineering Department, Universidade Federal do Rio Grande do Norte, Lagoa Nova, Natal, RN, 59078-900, Brazil.
Laboratory of Bioactive Compounds, Chemical Engineering Department, Universidade Federal do Rio Grande do Norte, Lagoa Nova, Natal, RN, 59078-900, Brazil.
Int Microbiol. 2024 Apr;27(2):513-523. doi: 10.1007/s10123-023-00412-7. Epub 2023 Jul 27.
The objective of this study was to investigate for the first time the role of S. cerevisiae natural barriers and endogenous cytoplasmatic bodies on the stabilization of fisetin encapsulated via sonoprocessing coupled to freeze-drying (FD) or spray drying (SD). Both protocols of encapsulation improved the resistance of fisetin against thermal treatments (between 60 and 150 °C) and photochemical-induced deterioration (light exposition for 60 days) compared to non-encapsulated fisetin (antioxidant activity retention of approximately 55% and 90%, respectively). When stored under constant relative humidity (from 32.8 to 90%) for 60 days, yeast carriers improved the half-life time of fisetin by up to 4-fold. Spray dried particles were smaller (4.9 μm) and showed higher fisetin release after simulated gastrointestinal digestion (55.7%) when compared to FD. Freeze-dried particles, in turn, tended to agglomerate more than SD (zeta potential -19.7 mV), resulting in reduced loading features (6.3 mg/g) and less efficient protection of fisetin to heat, photo, and moisture-induced deterioration. Overall, spray-dried sonoprocessed fisetin capsules are an efficient way to preserve fisetin against harsh conditions. Altogether, this report shows that sonoprocessing coupled to drying is an efficient, creative, and straightforward route to protect and deliver lipophilic fisetin using yeast capsules for food applications.
本研究旨在首次探讨酿酒酵母天然屏障和内细胞质体对通过声处理结合冷冻干燥（FD）或喷雾干燥（SD）包封的非瑟酮的稳定性的作用。与未包封的非瑟酮相比，两种包封方法均提高了非瑟酮对热处理（60-150°C）和光化学诱导降解（60 天光照暴露）的抗性（抗氧化活性保留率分别约为 55%和 90%）。在相对湿度恒定（32.8-90%）下储存 60 天时，酵母载体使非瑟酮的半衰期提高了 4 倍。与 FD 相比，喷雾干燥颗粒更小（4.9μm），并且在模拟胃肠道消化后具有更高的非瑟酮释放率（55.7%）。相反，冻干颗粒比 SD 更容易团聚（zeta 电位-19.7mV），导致负载特性降低（6.3mg/g），对热、光和水分诱导的降解的保护效率降低。总的来说，喷雾干燥声处理的非瑟酮胶囊是一种有效保存非瑟酮的方法，可以抵抗恶劣条件。总而言之，本报告表明，声处理结合干燥是一种有效、有创意且简单的方法，可使用酵母胶囊保护和输送亲脂性非瑟酮，用于食品应用。
