Bioprocess Laboratory, Chemical Engineering Department, Universidade Federal do Rio Grande do Norte, Lagoa Nova, Natal, RN, Brazil.
Laboratory of Bioactive Compounds, Chemical Engineering Department, Universidade Federal do Rio Grande do Norte, Lagoa Nova, Natal, RN, Brazil.
Lett Appl Microbiol. 2022 Dec;75(6):1538-1548. doi: 10.1111/lam.13820. Epub 2022 Sep 12.
Some of the challenges of yeast encapsulation protocols are low phytochemical internalization rates and limited intracellular compartments of yeasts. This study uses an ultrasound-assisted batch encapsulation (UABE) protocol to optimize the encapsulation of curcumin and fisetin by recovering nonencapsulated biomaterial and further incorporating it into nonloaded yeasts in three encapsulation stages (1ES, 2ES and 3ES). The effect of selected acoustic energies (166·7 and 333·3 W l ) on the encapsulation efficiency (EE), yield (EY) and antioxidant activity retention were evaluated, and then, compared with a control process (without ultrasound treatment). Compared to the control, enhanced EEs were achieved for both curcumin (10·9% control to 58·5% UABE) and fisetin (18·6% control to 76·6% UABE) after 3ES and the use of 333·3 W l . Similarly, the yeast maximum loading capacity was improved from 6·6 to 13·4 mg g for curcumin and from 11·1 to 26·4 mg g for fisetin after UABE protocol. The antioxidant activity of produced biocapsules was positively correlated with the bioactive-loaded content of yeasts when ultrasound treatment was applied. Overall, results from this study provide valuable information regarding UABE processes, and moreover, bring new and creative perspectives for ultrasound technology in the food industry.
一些酵母包封方案面临的挑战包括低植物化学物质内化率和酵母有限的细胞内隔室。本研究使用超声辅助批处理包封（UABE）方案，通过回收未包封的生物材料并进一步将其纳入三个包封阶段（1ES、2ES 和 3ES）的未负载酵母中，来优化姜黄素和非瑟酮的包封。选择了两种声能（166.7 和 333.3 W l ），评估其对包封效率（EE）、产率（EY）和抗氧化活性保留的影响，并与对照工艺（无超声处理）进行了比较。与对照相比，经过 3ES 和 333.3 W l 处理后，姜黄素的 EE 从 10.9%提高到 58.5%，非瑟酮的 EE 从 18.6%提高到 76.6%。同样，在使用 UABE 方案后，酵母的最大负载能力从 6.6 提高到 13.4 mg g ，对于姜黄素，从 11.1 提高到 26.4 mg g 。对于非瑟酮。当应用超声处理时，产生的生物胶囊的抗氧化活性与酵母中生物活性负载的含量呈正相关。总的来说，这项研究的结果提供了关于 UABE 工艺的有价值的信息，并且为超声技术在食品工业中的应用带来了新的和创造性的视角。
