Cardiovascular Research Center, Tabriz University of Medical Sciences, Tabriz, 5166615573, Iran.
Students Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
Mol Biol Rep. 2023 Sep;50(9):7527-7533. doi: 10.1007/s11033-023-08668-y. Epub 2023 Jul 27.
The slow coronary flow (SCF) phenomenon is considered a coronary artery disorder. Because of the critical function of peroxisome proliferator-activated receptors (PPARs) in regulating the oxidative stress and inflammatory reactions in cardiovascular disease, The aim of the current study was to investigate the expression of the genes for uncoupling proteins 1 and 2 (UCP1 and UCP2), peroxisome proliferator-activated receptors and (PPAR- PPAR-), and PPAR- in SCF patients.
In this case-control study, coronary angiography examination was used to analyze 35 individuals with SCF and 35 subjects with normal coronary flow (NCF). SCF was diagnosed using the TIMI (thrombolysis in myocardial infarction frame count) method. The SCF phenomenon is thought to be the TIMI > 27. In the peripheral blood mononuclear cells (PBMCs), the messenger ribonucleic acid (mRNA) expression levels of the PPAR-, PPAR-, UCP1, and UCP2 genes were evaluated.
UCP1 and UCP2 expression levels were significantly higher in the SCF group compared to the NCF group (P = 0.034 and P0.001, respectively). The PPAR- and PPAR- levels were found to be significantly lower in the SCF group compared to the NCF group (P = 0.015, P0.001, respectively). According to the results of the logistic regression analysis, high UCP1 and UCP2 levels and low PPAR- and PPAR- levels are each independent predictors of the SCF phenomenon.
This research provided evidence about the potential role of PPAR-α, PPAR-γ, UCP1, and UCP2 as biomarkers in SCF. More investigations are suggested to assess the functions of these factors in SCF patients mechanistically.
慢血流（SCF）现象被认为是一种冠状动脉疾病。由于过氧化物酶体增殖物激活受体（PPARs）在调节心血管疾病中的氧化应激和炎症反应方面具有重要作用，本研究旨在探讨解偶联蛋白 1 和 2（UCP1 和 UCP2）、过氧化物酶体增殖物激活受体和（PPAR-γ、PPAR-δ）以及 PPAR-γ 的基因在 SCF 患者中的表达。
在这项病例对照研究中，我们使用冠状动脉造影检查分析了 35 名 SCF 患者和 35 名正常冠状动脉血流（NCF）患者。SCF 采用 TIMI（心肌梗死溶栓帧数）法诊断。SCF 现象被认为是 TIMI>27。在外周血单核细胞（PBMCs）中，评估了 PPAR-γ、PPAR-δ、UCP1 和 UCP2 基因的信使核糖核酸（mRNA）表达水平。
与 NCF 组相比，SCF 组的 UCP1 和 UCP2 表达水平显著升高（P=0.034 和 P<0.001）。与 NCF 组相比，SCF 组的 PPAR-γ 和 PPAR-δ 水平显著降低（P=0.015、P<0.001）。根据逻辑回归分析的结果，高 UCP1 和 UCP2 水平以及低 PPAR-γ 和 PPAR-δ 水平均是 SCF 现象的独立预测因子。
本研究提供了证据表明 PPAR-α、PPAR-γ、UCP1 和 UCP2 作为 SCF 的潜在生物标志物。建议进行更多的研究来评估这些因素在 SCF 患者中的作用机制。
