Imam Mohamed S, Abdelazim Ahmed H, Ramzy Sherif, Batubara Afnan S, Gamal Mohammed, Abdelhafiz Safwan, Zeid Abdallah M
Pharmacy Practice Department, College of Pharmacy, Shaqra University, Shaqra, 11961, Saudi Arabia.
Clinical Pharmacy Department, National Cancer Institute, Cairo University, Fom El Khalig Square, Kasr Al-Aini Street, Cairo, 11796, Egypt.
BMC Chem. 2023 Jul 27;17(1):89. doi: 10.1186/s13065-023-01001-5.
The environmentally friendly design of analytical methods is gaining interest in pharmaceutical analysis to reduce hazardous environmental impacts and improve safety and health conditions for analysts. The adaptation and integration of chemometrics in the development of environmentally friendly analytical methods is strongly recommended in the hope of promising benefits. Favipiravir and remdesivir have been included in the COVID-19 treatment guidelines panel of several countries. The main objective of this work is to develop green, tuned spectrophotometric methods based on chemometric based models for the determination of favipiravir and remdesivir in spiked human plasma. The UV absorption spectra of favipiravir and remdesivir has shown overlap to some extent, making simultaneous determination difficult. Three advanced chemometric models, classical least squares, principal component regression, and partial least squares, have been developed to provide resolution and spectrophotometric determination of the drugs under study. A five-level, two-factor experimental design has been used to create the described models. The spectrally recorded data of favipiravir and remdesivir has been reviewed. The noise region has been neglected as it has a negative impact on the significant data. On the other hand, the other spectral data provided relevant information about the investigated drugs. A comprehensive evaluation and interpretation of the results of the described models and a statistical comparison with accepted values have been considered. The proposed models have been successfully applied to the spectrophotometric determination of favipiravir and remdesivir in pharmaceutical form spiked human plasma. In addition, the environmental friendliness of the described models was evaluated using the analytical eco-scale, the green analytical procedure index and the AGREE evaluation method. The results showed the compliance of the described models with the environmental characteristics.
分析方法的环保设计在药物分析领域正日益受到关注，旨在减少对环境的有害影响，并改善分析人员的安全与健康状况。强烈建议在环保分析方法的开发中采用并整合化学计量学，以期获得可观的效益。法匹拉韦和瑞德西韦已被纳入多个国家的新冠治疗指南专家组。本研究的主要目的是基于化学计量学模型开发绿色、优化的分光光度法，用于测定加标人血浆中法匹拉韦和瑞德西韦的含量。法匹拉韦和瑞德西韦的紫外吸收光谱在一定程度上存在重叠，使得同时测定变得困难。已开发了三种先进的化学计量学模型，即经典最小二乘法、主成分回归法和偏最小二乘法，以实现对所研究药物的分辨率和分光光度测定。采用五级二因素实验设计来构建所述模型。对法匹拉韦和瑞德西韦的光谱记录数据进行了审查。噪声区域因其对重要数据有负面影响而被忽略。另一方面，其他光谱数据提供了有关所研究药物的相关信息。已对所述模型的结果进行了全面评估和解释，并与公认值进行了统计比较。所提出的模型已成功应用于分光光度法测定加标人血浆中药物制剂中法匹拉韦和瑞德西韦的含量。此外，使用分析生态尺度、绿色分析程序指数和AGREE评估方法对所述模型的环境友好性进行了评估。结果表明所述模型符合环境特性。
