Guo Runying, Zhang Rui, Liu Sirui, Yang Yanyu, Dong Wenhang, Wang Meiyue, Mi Hongyan, Liu Mengzhe, Sun Jingjing, Zhang Xue, Su Yimeng, Liu Yiming, Huang Di, Li Rui
Department of Stomatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, PR China.
Department of Stomatology, The First Affiliated Hospital of Nanchang University, Nanchang 330000, PR China.
Regen Biomater. 2023 Jun 19;10:rbad061. doi: 10.1093/rb/rbad061. eCollection 2023.
It is still a huge challenge for bone regenerative biomaterial to balance its mechanical, biological and biodegradable properties. In the present study, a new composite material including treated dentin matrix (TDM) and α-calcium sulphate hemihydrate (α-CSH) was prepared. The optimal composition ratio between TDM and α-CSH was explored. The results indicate that both components were physically mixed and structurally stable. Its compressive strength reaches up to 5.027 ± 0.035 MPa for 50%TDM/α-CSH group, similar to human cancellous bone tissues. Biological experiments results show that TDM/α-CSH composite exhibits excellent biocompatibility and the expression of osteogenic related genes and proteins (ALP, RUNX2, OPN) is significantly increased. experiments suggest that the addition of TDM for each group (10%, 30%, 50%) effectively promotes cell proliferation and osteomalacia. In addition, 50% of the TDM/α-CSH combination displays optimal osteoconductivity. The novel TDM/α-CSH composite is a good candidate for certain applications in bone tissue engineering.
对于骨再生生物材料而言，平衡其机械性能、生物学性能和可生物降解性能仍然是一项巨大的挑战。在本研究中，制备了一种包含处理过的牙本质基质（TDM）和半水硫酸钙（α-CSH）的新型复合材料。探索了TDM与α-CSH之间的最佳组成比例。结果表明，两种成分物理混合且结构稳定。对于50%TDM/α-CSH组，其抗压强度高达5.027±0.035MPa，与人类松质骨组织相似。生物学实验结果表明，TDM/α-CSH复合材料表现出优异的生物相容性，并且成骨相关基因和蛋白质（碱性磷酸酶、RUNX2、骨桥蛋白）的表达显著增加。实验表明，每组（10%、30%、50%）添加TDM均能有效促进细胞增殖和骨软化。此外，50%的TDM/α-CSH组合表现出最佳的骨传导性。新型TDM/α-CSH复合材料是骨组织工程某些应用的良好候选材料。
