Chen I-Cheng, Su Chen-Ying, Lai Chun-Cheih, Tsou Yi-Syue, Zheng Yudong, Fang Hsu-Wei
Accelerator for Happiness and Health Industry, National Taipei University of Technology, Taipei 10608, Taiwan.
Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei 10608, Taiwan.
J Funct Biomater. 2021 Oct 4;12(4):56. doi: 10.3390/jfb12040056.
Demineralized bone matrix (DBM) is a decalcified allo/xenograft retaining collagen and noncollagenous proteins, which has been extensively used because of its osteoconductive and osteoinductive properties. Calcium sulfate (CaSO, CS) is a synthetic bone substitute used in bone healing with biocompatible, nontoxic, bioabsorbable, osteoconductive, and good mechanical characteristics. This study aims to prepare a DBM/CS composite bone graft material in a moldable putty form without compromising the peculiar properties of DBM and CS. For this purpose, firstly, porcine femur was defatted using chloroform/methanol and extracted by acid for demineralization, then freeze-dried and milled/sieved to obtain DBM powder. Secondly, the α-form and β-form of calcium sulfate hemihydrate (CaSO·0.5HO, CSH) were produced by heating gypsum (CaSO·2HO). The morphology and particle sizes of α- and β-CSH were obtained by SEM, and their chemical properties were confirmed by EDS, FTIR and XRD. Furthermore, the DBM-based graft was mixed with α- or β-CSH at a ratio of 9:1, and glycerol/4% HPMC was added as a carrier to produce a putty. DBM/CSH putty possesses a low washout rate, good mechanical strength and biocompatibility. In conclusion, we believe that the moldable DBM/CSH composite putty developed in this study could be a promising substitute for the currently available bone grafts, and might have practical application in the orthopedics field as a potential bone void filler.
脱矿骨基质（DBM）是一种保留了胶原蛋白和非胶原蛋白的脱钙同种/异种移植物，因其骨传导性和骨诱导性而被广泛应用。硫酸钙（CaSO₄，CS）是一种用于骨愈合的合成骨替代物，具有生物相容性、无毒、可生物吸收、骨传导性以及良好的力学特性。本研究旨在制备一种可塑形的油灰状DBM/CS复合骨移植材料，同时不损害DBM和CS的独特性能。为此，首先，用氯仿/甲醇对猪股骨进行脱脂，并用酸进行脱矿处理，然后冷冻干燥并研磨/筛分以获得DBM粉末。其次，通过加热石膏（CaSO₄·2H₂O）制备半水硫酸钙（CaSO₄·0.5H₂O，CSH）的α型和β型。通过扫描电子显微镜（SEM）获得α-CSH和β-CSH的形态和粒径，并通过能谱仪（EDS）、傅里叶变换红外光谱仪（FTIR）和X射线衍射仪（XRD）确认其化学性质。此外，将基于DBM的移植物与α-CSH或β-CSH按9:1的比例混合，并添加甘油/4%羟丙基甲基纤维素（HPMC）作为载体以制成油灰状材料。DBM/CSH油灰状材料具有低洗脱率、良好的机械强度和生物相容性。总之，我们认为本研究中开发出可塑形的DBM/CSH复合油灰状材料可能是现有骨移植材料的一种有前景的替代品，并且作为潜在的骨缺损填充材料可能在骨科领域有实际应用。
