Ji Lei, Jiang Tingting, Zhao Xin, Cai Damin, Hua Kouzhen, Du Peng, Chen Yuanyuan, Xie Jianping
School of Basic Medical Sciences and Forensic Medicine, Hangzhou Medical College, Hangzhou, Zhejiang, People's Republic of China.
Department of International Registration, Ustar Biotechnologies (Hangzhou) Ltd, Hangzhou, Zhejiang, People's Republic of China.
Infect Drug Resist. 2023 Jul 22;16:4755-4762. doi: 10.2147/IDR.S419914. eCollection 2023.
Fatty acid metabolism plays an important role in the survival and pathogenesis of . During dormancy, lipids are considered to be the main source of energy. A previous study found that Rv0494 is a starvation-inducible, lipid-responsive transcriptional regulator. However, the role of Rv0494 in bacterial persister survival has not been studied.
We constructed a deletion mutant strain of H37Rv and evaluated the susceptibility of the mutant strain to antibiotics using a persistence test.
We found that mutations in lead to survival defects of persisters, which reflected in increased sensitivity to isoniazid.
We conclude that Rv0494 is important for persister survival and may serve as a good target for developing new antibiotics that kill persister bacteria for improved treatment of persistent bacterial infections.
脂肪酸代谢在……的存活和发病机制中起重要作用。在休眠期间，脂质被认为是主要能量来源。先前的一项研究发现，Rv0494是一种饥饿诱导型、脂质反应性转录调节因子。然而，Rv0494在细菌持留菌存活中的作用尚未得到研究。
我们构建了H37Rv的缺失突变株，并使用持留菌测试评估突变株对抗生素的敏感性。
我们发现……中的突变导致持留菌的存活缺陷，这表现为对异烟肼的敏感性增加。
我们得出结论，Rv0494对持留菌的存活很重要，可能作为开发新型抗生素的良好靶点，这些抗生素可杀死持留菌，以改善对持续性细菌感染的治疗。
