Naranjo-Durán Ana María, Quintero-Quiroz Julián, Ciro-Gómez Gelmy Luz, Barona-Acevedo María-Jaqueline, Contreras-Calderón José de Carmen
Group of Toxinology, Food and Therapeutic Alternatives, College of Pharmaceutical and Food Sciences, University of Antioquia UdeA, Calle 67, 53-108, Medellin, Colombia.
College of Sciences and Biotechnology, CES University, Calle 10 # 22-04, Medellin, 050018, Colombia.
Heliyon. 2023 Jul 11;9(7):e17819. doi: 10.1016/j.heliyon.2023.e17819. eCollection 2023 Jul.
The consumption of antioxidants can prevent chronic non-communicable diseases and the exotic Colombian fruits, goldenberry (Physalis peruviana L.) and purple passion fruit (Passiflora edulis f. Edulis Sims), are rich in bioactive compounds. The aim of this work was to characterize and optimize the antioxidant activity of these fruits blend. The fruits were classified according to their maturity stages, the freeze-dried extracts were physiochemically characterized, and polyphenols, carotenoids and antioxidant activity were quantified, and an experimental mixture design was applied to optimize the antioxidant activity of the bend. For the goldenberry the maturity stage 3 had higher iron-reducing capacity and higher content of polyphenols. Meanwhile, for the purple passion fruit, this maturity stage had higher antioxidant activity by all methodologies and a higher concentration of polyphenols; the ultrasound-assisted extraction showed statistical differences for polyphenols, ABTS and FRAP. Antioxidant activity showed significant differences (p < 0.05) between samples (TBARS (3.98 ± 0.14 and 7.03 ± 0.85 μM-MDA/g), ABTS (36.53 ± 2.66 and 29.4 ± 4.88 μMTrolox/g), DPPH (36.53 ± 2.66 and 23.90 ± 0.96μMTrolox/g), ORAC (23.02 ± 0.36 and 32.44 ± 0.94 μM Trolox/g) and total polyphenols (5, 29 ± 0.34 and 9.12 ± 0.37mgGA/g). Some of the carotenoids identified by HPLC-MS in both fruits were lutein, α and β-carotene, phytoene and lycopene. The optimum bend was goldenberry 0.83 and purple passion fruit 0.17.
食用抗氧化剂可预防慢性非传染性疾病，而哥伦比亚特色水果灯笼果（酸浆属酸浆）和紫果西番莲富含生物活性化合物。本研究旨在对这些水果混合物的抗氧化活性进行表征和优化。根据水果的成熟阶段进行分类，对冻干提取物进行理化特性分析，对多酚、类胡萝卜素和抗氧化活性进行定量，并采用实验混合物设计来优化混合物的抗氧化活性。对于灯笼果，成熟阶段3具有更高的铁还原能力和更高的多酚含量。同时，对于紫果西番莲，该成熟阶段通过所有方法测定均具有更高的抗氧化活性和更高的多酚浓度；超声辅助提取在多酚、ABTS和FRAP方面显示出统计学差异。样品之间的抗氧化活性存在显著差异（p < 0.05）（硫代巴比妥酸反应物（3.98±0.14和7.03±0.85 μM - 丙二醛/克）、ABTS（36.53±2.66和29.4±4.88 μM Trolox/克）、DPPH（36.53±2.66和23.90±0.96 μM Trolox/克）、氧自由基吸收能力（23.02±0.36和32.44±0.94 μM Trolox/克）以及总多酚（5.29±0.34和9.12±0.37毫克没食子酸/克））。通过高效液相色谱 - 质谱法在两种水果中鉴定出的一些类胡萝卜素为叶黄素、α和β - 胡萝卜素、八氢番茄红素和番茄红素。最佳混合物比例为灯笼果0.83和紫果西番莲0.17。
