Quintero Quiroz Julian, Naranjo Duran Ana Maria, Silva Garcia Mariluz, Ciro Gomez Gelmy Luz, Rojas Camargo John Jairo
College of Pharmaceutical and Food Sciences, University of Antioquia, Street 67, No. 53-108, Medellin, Colombia.
Institute of Food Science and Technology (INTAL), Carrera 50g No. 12S-91, Itagüi, Colombia.
Int J Food Sci. 2019 Jul 16;2019:3721828. doi: 10.1155/2019/3721828. eCollection 2019.
This study evaluated the antimicrobial activity (i.e., against and ) and the antioxidant activity (i.e., ABTS, FRAP, and DPPH) of annatto seeds extract obtained by ultrasound-assisted extraction. A response surface design with three levels such as pH (2-11), solvent concentration (50-96 %), seed-to-solvent ratio (1:2-1:10), and treatment time (0-30 min) was employed to determine the optimal experimental conditions. Thus, a pH of 7.0, seed-to-solvent ratio of 1:7, and treatment time of 20 min were selected as optimal rendering an extract having a 0.62% of bixin, 3.81 mg gallic acid/mg equivalent of polyphenol compounds (ABTS 1035.7, FRAP 424.7, and DPPH 1161.5 M trolox/L), and a minimal inhibitory concentration against and of 32 and 16 mg/L, respectively. Further, the main bioactive compounds identified by LC/ESI-MS were bixin and catechin, chlorogenic acid, chrysin, butein, hypolaetin, licochalcone A, and xanthohumol.
本研究评估了通过超声辅助提取获得的胭脂树籽提取物的抗菌活性（即针对 和 ）和抗氧化活性（即ABTS、FRAP和DPPH）。采用具有三个水平的响应面设计，如pH值（2 - 11）、溶剂浓度（50 - 96%）、籽与溶剂比例（1:2 - 1:10）和处理时间（0 - 30分钟），以确定最佳实验条件。因此，选择pH值7.0、籽与溶剂比例1:7和处理时间20分钟作为最佳条件，得到的提取物含有0.62%的胭脂树橙、3.81毫克没食子酸/毫克当量的多酚化合物（ABTS为1035.7、FRAP为�24.7、DPPH为1161.5微摩尔Trolox/升），对 和 的最小抑菌浓度分别为32和16毫克/升。此外，通过LC/ESI-MS鉴定的主要生物活性化合物为胭脂树橙和儿茶素、绿原酸、白杨素、布枯叶素、异槲皮素、甘草查尔酮A和黄腐酚。
