Department of Fruit, Vegetable and Nutraceutical Plant Technology, Wrocław University of Environmental and Life Sciences, 37 Chełmońskiego Street, 51-630 Wrocław, Poland.
Molecules. 2023 Sep 20;28(18):6711. doi: 10.3390/molecules28186711.
A polyphenolic preparation in the form of the passion fruit epicarp extract was analyzed to identify and quantify the polyphenolic compounds using LC QTOF/ESI-MS and UPLC-PDA-FL. The analyzed parameters included antidiabetic activity (α-amylase, α-glucosidase, and pancreatic lipase), inhibitory activity toward cholinesterase (AChE, BuChE), anti-inflammatory activity (COX-1, COX-2, 15-LOX) and antioxidant activity based on ORAC and ABTS. The polyphenolic preparation of the passion fruit epicarp extract contained 51 polyphenolic compounds representing five groups-flavones (25 compounds; 52% of total polyphenolic), flavonols (8; 16%), flavan-3-ols (6; 7%), phenolic acids (4; 3%), and anthocyanins (7; 21%), with derivatives of luteolin (13 derivatives) and apigenin (8 derivatives) as dominant compounds. The preparation was characterized by an antioxidant activity of 160.7 (ORAC) and 1004.4 mmol Trolox/100 mL (ABTS). The inhibitory activity toward α-amylase, α-glucosidase, and pancreatic lipase reached IC of 7.99, 12.80, and 0.42, respectively. The inhibition of cholinesterases (IC) was 18.29 for AChE and 14.22 for BuChE. Anti-inflammatory activity as IC was 6.0 for COX-1, 0.9 for COX-2, and 4.9 for 15-LOX. Food enriched with passion fruit epicarp extract has a potentially therapeutic effect.
一种以百香果果皮提取物形式存在的多酚制剂，通过 LC QTOF/ESI-MS 和 UPLC-PDA-FL 进行分析，以鉴定和定量多酚化合物。分析的参数包括抗糖尿病活性（α-淀粉酶、α-葡萄糖苷酶和胰脂肪酶）、对胆碱酯酶（AChE、BuChE）的抑制活性、抗炎活性（COX-1、COX-2、15-LOX）和基于 ORAC 和 ABTS 的抗氧化活性。百香果果皮提取物的多酚制剂含有 51 种多酚化合物，代表五个组-黄酮类（25 种化合物；占总多酚的 52%）、黄酮醇（8 种）、黄烷-3-醇（6 种）、酚酸（4 种）和花色苷（7 种；21%），以木犀草素（13 种衍生物）和芹菜素（8 种衍生物）为主要化合物。该制剂的抗氧化活性为 160.7（ORAC）和 1004.4mmol Trolox/100mL（ABTS）。对 α-淀粉酶、α-葡萄糖苷酶和胰脂肪酶的抑制活性分别达到 7.99、12.80 和 0.42 的 IC。对乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BuChE）的抑制作用分别为 18.29 和 14.22 的 IC。抗炎活性的 IC 为 6.0 的 COX-1、0.9 的 COX-2 和 4.9 的 15-LOX。富含百香果果皮提取物的食物具有潜在的治疗效果。
