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关于5-溴尿嘧啶掺入导致的DNA损伤的性质及切除修复的遗传学证据。

Genetic evidence for the nature, and excision repair, of DNA lesions resulting from incorporation of 5-bromouracil.

作者信息

Krych M, Pietrzykowska I, Szyszko J, Shugar D

出版信息

Mol Gen Genet. 1979 Mar 20;171(2):135-43. doi: 10.1007/BF00269999.

DOI:10.1007/BF00269999
PMID:375026
Abstract

Escherichia coli mutants defective in DNA uracil N-glycosidase (ung-) or endonuclease VI active against apurinic/apyrimidinic sites in DNA (xthA-) exhibit enhanced sensitivity towards 5-bromodeoxyuridine relative to the wild type strain, pointing to involvement of these enzymes in repair of bromouracil-induced lesions in DNA. Mutants defective in DNA polymerase I, either in polymerizing activity (polAl-) or (5' leads to 3')-exonuclease activity (polA107-) exhibit unusually high sensitivity (including marked lethality) in the presence of 5-bromodeoxyuridine. The results indicate that DNA polymerase I, and its associated (5'--3')-exonuclease activity, are involved in repair of bromouracil-induced lesions and are not readily replaced, if at all, by DNA polymerases II and III. Thermosensitive mutant in DNA ligase gene (lig ts7) shows high sensitivity towards 5-bromodeoxyuridine at 42 degrees C indicating the role of the enzyme in repair of bromouracil-induced lesions in DNA. Involvement of DNA uracil N-glycosidase, and endonuclease active against apurinic/apyrimidinic sites in recognition and repair of 5-bromouracil-induced damage permits of some inferences regarding the nature of this damage (lesions), in particular dehalogenation of incorporated bromouracil to uracil residues.

摘要

与野生型菌株相比，DNA尿嘧啶N-糖苷酶（ung-）或对DNA中脱嘌呤/脱嘧啶位点有活性的核酸内切酶VI（xthA-）缺陷的大肠杆菌突变体对5-溴脱氧尿苷表现出更高的敏感性，这表明这些酶参与了DNA中溴尿嘧啶诱导损伤的修复。DNA聚合酶I在聚合活性（polA1-）或（5'→3'）外切核酸酶活性（polA107-）方面有缺陷的突变体，在存在5-溴脱氧尿苷的情况下表现出异常高的敏感性（包括明显的致死性）。结果表明，DNA聚合酶I及其相关的（5'→3'）外切核酸酶活性参与了溴尿嘧啶诱导损伤的修复，并且如果可以被DNA聚合酶II和III替代的话，也不容易被替代。DNA连接酶基因的温度敏感突变体（lig ts7）在42℃时对5-溴脱氧尿苷表现出高敏感性，表明该酶在DNA中溴尿嘧啶诱导损伤的修复中起作用。DNA尿嘧啶N-糖苷酶和对脱嘌呤/脱嘧啶位点有活性的核酸内切酶参与5-溴尿嘧啶诱导损伤的识别和修复，这使得我们可以对这种损伤（病变）的性质做出一些推断，特别是掺入的溴尿嘧啶脱卤化为尿嘧啶残基。

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本文引用的文献

1
Mutants of Escherichia coli K-12 defective in DNA repair and in genetic recombination.大肠杆菌K-12中DNA修复和基因重组存在缺陷的突变体。
Genetics. 1966 Jun;53(6):1137-50. doi: 10.1093/genetics/53.6.1137.
2
Genetic analysis of an E. coli strain with a mutation affecting DNA polymerase.对一株具有影响DNA聚合酶突变的大肠杆菌菌株进行基因分析。
Nature. 1969 Dec 20;224(5225):1166-8. doi: 10.1038/2241166a0.
3
On the mechanism of bromouracil-induced mutagenesis.
Mutat Res. 1973 Jul;19(1):1-9. doi: 10.1016/0027-5107(73)90107-3.
Mol Gen Genet. 1983;191(2):276-81. doi: 10.1007/BF00334826.
4
Evidence implying DNA polymerase beta function in excision repair.有证据表明DNA聚合酶β在切除修复中发挥作用。
Nucleic Acids Res. 1980 Jan 25;8(2):361-75. doi: 10.1093/nar/8.2.361.
5
Pairing properties of bromouracil and repair of bromouracil-containing DNA. Possible utilization of bromodeoxyuridine triphosphate for site-directed mutagenesis.溴尿嘧啶的配对特性及含溴尿嘧啶DNA的修复。三磷酸溴脱氧尿苷用于定点诱变的可能性。
Biochem J. 1988 Aug 1;253(3):637-43. doi: 10.1042/bj2530637.
4
Debromination of 5-bromo-2'-deoxyuridine by cysteine. Formation of deoxyuridine and S-(5-(2'-deoxyuridyl))cysteine.半胱氨酸对5-溴-2'-脱氧尿苷的脱溴作用。脱氧尿苷和S-(5-(2'-脱氧尿苷基))半胱氨酸的形成。
Biochemistry. 1973 Sep 25;12(20):3992-8. doi: 10.1021/bi00744a032.
5
An N-glycosidase from Escherichia coli that releases free uracil from DNA containing deaminated cytosine residues.一种来自大肠杆菌的N-糖苷酶，可从含有脱氨基胞嘧啶残基的DNA中释放游离尿嘧啶。
Proc Natl Acad Sci U S A. 1974 Sep;71(9):3649-53. doi: 10.1073/pnas.71.9.3649.
6
The enzymatic release of O6-methylguanine and 3-methyladenine from DNA reacted with the carcinogen N-methyl-N-nitrosourea.与致癌物N-甲基-N-亚硝基脲反应的DNA中O6-甲基鸟嘌呤和3-甲基腺嘌呤的酶促释放。
Proc Natl Acad Sci U S A. 1974 May;71(5):2022-6. doi: 10.1073/pnas.71.5.2022.
7
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8
Involvement of DNA polymerase 3 in excision repair after ultraviolet irradiation.DNA聚合酶3参与紫外线照射后的切除修复。
Nat New Biol. 1973 Aug 22;244(138):240-1. doi: 10.1038/newbio244240a0.
9
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J Bacteriol. 1973 Apr;114(1):121-7. doi: 10.1128/jb.114.1.121-127.1973.
10
Conditional lethality of recA and recB derivatives of a strain of Escherichia coli K-12 with a temperature-sensitive deoxyribonucleic acid polymerase I.大肠杆菌K-12菌株的recA和recB衍生物与温度敏感型脱氧核糖核酸聚合酶I的条件致死性
J Bacteriol. 1972 Mar;109(3):971-8. doi: 10.1128/jb.109.3.971-978.1972.