一种来自大肠杆菌的核酸内切酶，它优先作用于紫外线照射过的DNA，并且在uvrA和uvrB突变体中不存在。
An endonuclease from Escherichia coli that acts preferentially on UV-irradiated DNA and is absent from the uvrA and uvrB mutants.
作者信息
Braun A, Grossman L
出版信息
Proc Natl Acad Sci U S A. 1974 May;71(5):1838-42. doi: 10.1073/pnas.71.5.1838.
Abstract
At least two endonucleolytic activities that preferentially incise ultraviolet (UV)-irradiated DNA exist in extracts of E. coli. These two activities can be separated by phosphocellulose chromatographic fractionation. The subject of this paper is one of these activities, which elutes from phosphocellulose with 0.25 M potassium phosphate, pH 7.5. This endonucleolytic activity specific for UV-irradiated DNA is absent from partially purified extracts of uvrA and uvrB mutants, which are defective in excision of pyrimidine dimers, but is present in normal amounts in the uvrC excision-defective mutant. The enzyme binds very tightly and specifically to UV-irradiated DNA. Binding can be prevented by prior treatment of the irradiated DNA with photoreactivating enzyme. This binding activity is absent in uvrA and uvrB mutants, but present in uvrC and uvrD mutants.
摘要
大肠杆菌提取物中存在至少两种优先切割紫外线（UV）照射过的DNA的核酸内切酶活性。这两种活性可以通过磷酸纤维素柱层析分离。本文的研究对象是其中一种活性，它在pH 7.5的0.25 M磷酸钾条件下从磷酸纤维素上洗脱下来。这种对紫外线照射过的DNA具有特异性的核酸内切酶活性在嘧啶二聚体切除存在缺陷的uvrA和uvrB突变体的部分纯化提取物中不存在，但在uvrC切除缺陷突变体中含量正常。该酶与紫外线照射过的DNA紧密且特异性结合。用光复活酶预先处理照射过的DNA可阻止这种结合。uvrA和uvrB突变体中不存在这种结合活性，但uvrC和uvrD突变体中存在。
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An endonuclease from Escherichia coli that acts preferentially on UV-irradiated DNA and is absent from the uvrA and uvrB mutants.一种来自大肠杆菌的核酸内切酶，它优先作用于紫外线照射过的DNA，并且在uvrA和uvrB突变体中不存在。
Proc Natl Acad Sci U S A. 1974 May;71(5):1838-42. doi: 10.1073/pnas.71.5.1838.
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本文引用的文献
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Three loci in Escherichia coli K-12 that control the excision of pyrimidine dimers and certain other mutagen products from DNA.大肠杆菌K-12中控制从DNA切除嘧啶二聚体和某些其他诱变产物的三个基因座。
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Spectral properties of the chromophoric material associated with the deoxyribonucleic acid photoreactivating enzyme isolated from baker's yeast.从面包酵母中分离出的与脱氧核糖核酸光复活酶相关的发色物质的光谱特性。
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An endonuclease induced after infection of Escherichia coli with bacteriophage T7. I. Purification and properties of the enzyme.噬菌体T7感染大肠杆菌后诱导产生的一种核酸内切酶。I. 该酶的纯化及性质
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