Tan N H, Kanthimathi M S, Tan C S
Toxicon. 1986;24(6):626-30. doi: 10.1016/0041-0101(86)90186-8.
The enzyme contents of four venom samples of Calloselasma rhodostoma were analyzed. The venoms contained phosphodiesterase, alkaline phosphomonoesterase, 5'-nucleotidase, protease, phospholipase A, L-amino acid oxidase, hyaluronidase, arginine ester hydrolase, arginine amidase, fibrinogenase and coagulant enzyme activities. There is significant variation in the contents of coagulant enzyme, arginine ester hydrolase, hyaluronidase, protease, phosphodiesterase, alkaline phosphomonoesterase and L-amino acid oxidase. DEAE-Sephacel ion exchange chromatography of the venom resolved it into eight major protein fractions. The eight fractions were heterogeneous and exhibited more than one type of enzymatic activity. The 5'-nucleotidase, alkaline phosphomonoesterase, protease, coagulant enzyme, arginine ester hydrolase, arginine amidase and fibrinogenase exist in multiple forms.
对圆斑蝰四个毒液样本的酶含量进行了分析。毒液含有磷酸二酯酶、碱性磷酸单酯酶、5'-核苷酸酶、蛋白酶、磷脂酶A、L-氨基酸氧化酶、透明质酸酶、精氨酸酯水解酶、精氨酸酰胺酶、纤维蛋白原酶和凝血酶活性。凝血酶、精氨酸酯水解酶、透明质酸酶、蛋白酶、磷酸二酯酶、碱性磷酸单酯酶和L-氨基酸氧化酶的含量存在显著差异。毒液经DEAE-葡聚糖离子交换层析可分离为八个主要蛋白质组分。这八个组分不均一，表现出不止一种类型的酶活性。5'-核苷酸酶、碱性磷酸单酯酶、蛋白酶、凝血酶、精氨酸酯水解酶、精氨酸酰胺酶和纤维蛋白原酶以多种形式存在。
