Lersten N R
Stain Technol. 1986 Jul;61(4):231-4. doi: 10.3109/10520298609109942.
Leaf samples of Glycine max and numerous other dicotyledonous species were cleared by a common, well established procedure modified by using more concentrated (10% w/v) aqueous NaOH, and by leaving samples in NaOH for 2-4 weeks and in chloral hydrate for 3 days, all at room temperature. A single dye, chlorazol black E (1 g/100 ml absolute ethanol), is used to stain for 3-6 min. Samples are mounted with the lower epidermis upward. Sieve tubes in favorable material can be seen in minor veins and vein endings.
通过一种常用的、成熟的程序对大豆和许多其他双子叶植物的叶片样本进行透明处理，该程序经过修改，使用了浓度更高（10% w/v）的氢氧化钠水溶液，将样本在氢氧化钠中放置2至4周，在水合氯醛中放置3天，所有操作均在室温下进行。使用单一染料氯唑黑E（1 g/100 ml无水乙醇）进行染色3至6分钟。样本以较低表皮向上的方式进行封片。在有利材料中的筛管可在细脉和叶脉末端看到。
