Henan Key Laboratory of Biomolecular Recognition and Sensing, College of Chemistry and Chemical Engineering, Henan Joint International Research Laboratory of Chemo/Biosensing and Early Diagnosis of Major Diseases, Shangqiu Normal University, Shangqiu, 476000, Henan Province, PR China.
Polymer Biointerface Centre, School of Chemical Sciences, The University of Auckland, Private Bag 92019, Auckland, 1142, New Zealand; MacDiarmid Institute for Advanced Materials and Nanotechnology, Victoria University of Wellington, PO Box 600, Wellington, New Zealand.
Biosens Bioelectron. 2021 Aug 1;185:113247. doi: 10.1016/j.bios.2021.113247. Epub 2021 Apr 28.
17β-Estradiol (E2), the strongest of the three major physiological estrogens in females, is an important factor in the female reproductive system. The abnormal level of E2 causes health issues, such as weak bones, urinary tract infections and even depression. Here, we present a novel, sensitive and selective, electrochemical aptasensor for detection of 17β-estradiol (E2). The E2 recognition aptamer was split into two fragments: the first fragment, functionalised with adamantane, is attached to poly(β-cyclodextrin) (poly(β-CD))-modified electrode surface through host-guest interactions between the adamantane and poly(β-CD). The second fragment, labelled with gold nanoparticles, forms the stem-loop structure with the first fragment only in the presence of E2. That specific recognition process triggers the change in the electrochemical signal (a change in the peak current from reduction of AuNPs), recorded by means of differential pulse voltammetry (DPV). The feasibility of the sensing design was firstly investigated on the commercially available glass carbon electrodes (GCE), with achieved a linear detection range of 1.0 × 10 to 1.0 × 10 M and a limit of detection (LoD) 0.7 fM. The sensing methodology was then translated onto single-use, disposable, laser-scribed graphene electrodes (LSGE) on a plastic substrate. The dynamic sensing range of E2 on LSGE was found to be 1.0 × 10 to 1.0 × 10 M, with a LoD of 63.1 fM, comparable to these of GCE. The successful translation of the developed E2 aptasensor from GCE to low-cost, disposable LSGE highlights a potential of this sensing platform in commercial, portable sensing detection systems for E2 and similar targets of biological interest.
17β-雌二醇（E2）是女性三种主要生理雌激素中最强的一种，是女性生殖系统的重要因素。E2 水平异常会导致健康问题，如骨质疏松、尿路感染，甚至抑郁。在这里，我们提出了一种新颖、灵敏和选择性的电化学适体传感器，用于检测 17β-雌二醇（E2）。E2 识别适体被分裂成两个片段：第一个片段，功能化的金刚烷，通过金刚烷和聚（β-环糊精）（poly(β-CD)）之间的主体-客体相互作用附着在聚（β-CD）修饰的电极表面上。第二个片段，用金纳米粒子标记，仅在存在 E2 的情况下与第一个片段形成茎环结构。这种特定的识别过程触发电化学信号的变化（来自 AuNPs 还原的峰电流变化），通过差分脉冲伏安法（DPV）记录。传感设计的可行性首先在市售的玻碳电极（GCE）上进行了研究，实现了 1.0×10 到 1.0×10 的线性检测范围和 0.7 fM 的检测限（LoD）。然后将传感方法转化到在塑料基底上的一次性、一次性使用的激光刻蚀石墨烯电极（LSGE）上。发现 E2 在 LSGE 上的动态传感范围为 1.0×10 到 1.0×10，检测限（LoD）为 63.1 fM，与 GCE 的相当。从 GCE 成功地将开发的 E2 适体传感器转化为低成本、一次性使用的 LSGE，突出了该传感平台在商业、便携式 E2 和类似生物感兴趣目标的传感检测系统中的潜力。
