Embrapa Recursos Genéticos e Biotecnologia, Laboratório de Tecnologias para a Segurança Alimentar (LSA), Parque Estação Biológica, Final W5 Norte, Brasília 70770-917, DF, Brazil.
Embrapa Recursos Genéticos e Biotecnologia, Laboratório de Nanobiotecnologia (LNANO), Parque Estação Biológica, Final W5 Norte, Brasília 70770-917, DF, Brazil.
Biosensors (Basel). 2023 Jul 21;13(7):751. doi: 10.3390/bios13070751.
The global consumption of vegan foods is experiencing an expressive upward trend, underscoring the critical need for quality control measures based on nutritional and functional considerations. This study aimed to evaluate the functional quality of caviar and salmon analog food inks based on pulses combined with nano ingredients and produced in our laboratory (LNANO). The primary objective of this work was to determine the total antioxidant compounds contained in these samples using a voltammetric technique with a glassy carbon electrode. The samples underwent ethanolic extraction (70%) with 1 h of stirring. The voltammograms were acquired in a phosphate buffer electrolyte, pH 3.0 with Ag/AgCl (KCl 3 mol L) as the reference electrode and platinum wire as the auxiliary electrode. The voltammograms revealed prominent anodic current peaks at 0.76-0.78 V, which are attributed to isoflavones. Isoflavones, known secondary metabolites with substantial antioxidant potential commonly found in pulses, were identified. The total isoflavone concentrations obtained ranged from 31.5 to 64.3 mg Eq genistein 100 g. The results not only validated the efficacy of the electrochemical sensor for quantifying total antioxidant compounds in the samples but also demonstrated that the concentration of total isoflavones in caviar and salmon analogs fell within the expected limits.
全球范围内对纯素食品的消费呈显著上升趋势，这凸显了基于营养和功能考虑进行质量控制措施的迫切需求。本研究旨在评估基于豆类与纳米成分结合并在我们实验室（LNANO）生产的鱼子酱和三文鱼模拟食品墨水的功能质量。这项工作的主要目的是使用带有玻碳电极的伏安技术来测定这些样品中的总抗氧化化合物。样品经过 70%乙醇提取（1 h 搅拌）。在 pH 值为 3.0 的磷酸盐缓冲电解液中获取伏安图，以 Ag/AgCl（KCl 3 mol L）作为参比电极，铂丝作为辅助电极。伏安图在 0.76-0.78 V 处显示出明显的阳极电流峰，这归因于异黄酮。异黄酮是豆类中常见的具有大量抗氧化潜力的次生代谢物，已被鉴定出来。获得的总异黄酮浓度范围为 31.5 至 64.3 mg Eq 染料木黄酮/100 g。研究结果不仅验证了电化学传感器用于定量样品中总抗氧化化合物的有效性，还表明鱼子酱和三文鱼模拟品中的总异黄酮浓度在预期范围内。
