Key Laboratory of Biomedical Engineering of Hainan Province, School of Biomedical Engineering, Hainan University, Haikou 570228, China.
One Health Institute, Hainan University, Haikou 570228, China.
Biosensors (Basel). 2023 Jul 21;13(7):752. doi: 10.3390/bios13070752.
Leucine aminopeptidase (LAP) is an important protease that can specifically hydrolyze Leucine residues. LAP occurs in microorganisms, plants, animals, and humans and is involved in a variety of physiological processes in the human body. In the physiological system, abnormal levels of LAP are associated with a variety of diseases and pathological processes, such as cancer and drug-induced liver injury; thus, LAP was chosen as the early biochemical marker for many physiological processes, including cancer. Considering the importance of LAP in physiological and pathological processes, it is critical that high-efficiency and dependable technology be developed to monitor LAP levels. Herein, we summarize the organic small molecule fluorescence/chemiluminescence probes used for LAP detection in recent years, which can image LAP in cancer, drug-induced liver injury (DILI), and bacteria. It can also reveal the role of LAP in tumors and differentiate the serum of cirrhotic, drug-induced liver injury and normal models.
亮氨酸氨基肽酶（LAP）是一种重要的蛋白酶，能够特异性水解亮氨酸残基。LAP 存在于微生物、植物、动物和人类中，参与人体的多种生理过程。在生理系统中，LAP 水平异常与多种疾病和病理过程有关，如癌症和药物性肝损伤；因此，LAP 被选为许多生理过程（包括癌症）的早期生化标志物。鉴于 LAP 在生理和病理过程中的重要性，开发高效、可靠的技术来监测 LAP 水平至关重要。在此，我们总结了近年来用于 LAP 检测的有机小分子荧光/化学发光探针，这些探针可用于癌症、药物性肝损伤（DILI）和细菌的 LAP 成像，还可以揭示 LAP 在肿瘤中的作用，并区分肝硬化、药物性肝损伤和正常模型的血清。
Zotero 插件
