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基于活性的荧光探针用于亮氨酸氨肽酶成像的最新进展。

Recent Progress of Activity-Based Fluorescent Probes for Imaging Leucine Aminopeptidase.

机构信息

Key Laboratory of Biomedical Engineering of Hainan Province, School of Biomedical Engineering, Hainan University, Haikou 570228, China.

One Health Institute, Hainan University, Haikou 570228, China.

出版信息

Biosensors (Basel). 2023 Jul 21;13(7):752. doi: 10.3390/bios13070752.

DOI:10.3390/bios13070752
PMID:37504150
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10377407/
Abstract

Leucine aminopeptidase (LAP) is an important protease that can specifically hydrolyze Leucine residues. LAP occurs in microorganisms, plants, animals, and humans and is involved in a variety of physiological processes in the human body. In the physiological system, abnormal levels of LAP are associated with a variety of diseases and pathological processes, such as cancer and drug-induced liver injury; thus, LAP was chosen as the early biochemical marker for many physiological processes, including cancer. Considering the importance of LAP in physiological and pathological processes, it is critical that high-efficiency and dependable technology be developed to monitor LAP levels. Herein, we summarize the organic small molecule fluorescence/chemiluminescence probes used for LAP detection in recent years, which can image LAP in cancer, drug-induced liver injury (DILI), and bacteria. It can also reveal the role of LAP in tumors and differentiate the serum of cirrhotic, drug-induced liver injury and normal models.

摘要

亮氨酸氨基肽酶（LAP）是一种重要的蛋白酶，能够特异性水解亮氨酸残基。LAP 存在于微生物、植物、动物和人类中，参与人体的多种生理过程。在生理系统中，LAP 水平异常与多种疾病和病理过程有关，如癌症和药物性肝损伤；因此，LAP 被选为许多生理过程（包括癌症）的早期生化标志物。鉴于 LAP 在生理和病理过程中的重要性，开发高效、可靠的技术来监测 LAP 水平至关重要。在此，我们总结了近年来用于 LAP 检测的有机小分子荧光/化学发光探针，这些探针可用于癌症、药物性肝损伤（DILI）和细菌的 LAP 成像，还可以揭示 LAP 在肿瘤中的作用，并区分肝硬化、药物性肝损伤和正常模型的血清。

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