State Key Laboratory of Chemical Resource Engineering, College of Chemistry, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing, 100029, China.
Department of Chemistry, University of Bath, Bath BA2 7AY, UK.
Chem Soc Rev. 2023 Jun 6;52(11):3873-3926. doi: 10.1039/d2cs00172a.
Oxidative stress is closely related to the physiopathology of numerous diseases. Reactive oxygen species (ROS), reactive nitrogen species (RNS), and reactive sulfur species (RSS) are direct participants and important biomarkers of oxidative stress. A comprehensive understanding of their changes can help us evaluate disease pathogenesis and progression and facilitate early diagnosis and drug development. In recent years, fluorescent probes have been developed for real-time monitoring of ROS, RNS and RSS levels and . In this review, conventional design strategies of fluorescent probes for ROS, RNS, and RSS detection are discussed from three aspects: fluorophores, linkers, and recognition groups. We introduce representative fluorescent probes for ROS, RNS, and RSS detection in cells, physiological/pathological processes (, Inflammation, Drug Induced Organ Injury and Ischemia/Reperfusion Injury ), and specific diseases (, neurodegenerative diseases, epilepsy, depression, diabetes and cancer, ). We then highlight the achievements, current challenges, and prospects for fluorescent probes in the pathophysiology of oxidative stress-related diseases.
氧化应激与许多疾病的病理生理学密切相关。活性氧（ROS）、活性氮（RNS）和活性硫（RSS）是氧化应激的直接参与者和重要生物标志物。全面了解它们的变化有助于我们评估疾病的发病机制和进展，并促进早期诊断和药物开发。近年来，已经开发出用于实时监测 ROS、RNS 和 RSS 水平的荧光探针。在这篇综述中，我们从荧光团、连接子和识别基团三个方面讨论了用于 ROS、RNS 和 RSS 检测的荧光探针的传统设计策略。我们介绍了用于 ROS、RNS 和 RSS 在细胞内、生理/病理过程（炎症、药物诱导的器官损伤和缺血/再灌注损伤）以及特定疾病（神经退行性疾病、癫痫、抑郁、糖尿病和癌症）检测的代表性荧光探针。然后，我们重点介绍了荧光探针在氧化应激相关疾病病理生理学中的成就、当前挑战和前景。
