探索七种消化系统癌症的关键生物标志物、共同发病机制及其与COVID-19的相关性。

Exploring Key Biomarkers and Common Pathogenesis of Seven Digestive System Cancers and Their Correlation with COVID-19.

作者信息

Xiong Zuming, Yang Yongjun, Li Wenxin, Lin Yirong, Huang Wei, Zhang Sen

机构信息

Department of Colorectal and Anal Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China.

出版信息

Curr Issues Mol Biol. 2023 Jun 30;45(7):5515-5533. doi: 10.3390/cimb45070349.

DOI:10.3390/cimb45070349

PMID:37504265

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10378662/

Abstract

Digestive system cancer and COVID-19 significantly affect the digestive system, but the mechanism of interaction between COVID-19 and the digestive system cancers has not been fully elucidated. We downloaded the gene expression of COVID-19 and seven digestive system cancers (oral, esophageal, gastric, colorectal, hepatocellular, bile duct, pancreatic) from GEO and identified hub differentially expressed genes. Multiple verifications, diagnostic efficacy, prognostic analysis, functional enrichment and related transcription factors of hub genes were explored. We identified 23 common DEGs for subsequent analysis. CytoHubba identified nine hub genes (, , , , , , , , ). TCGA and GEO data validated the expression and excellent diagnostic and prognostic ability of hub genes. Functional analysis revealed that the processes of cell division and the cell cycle were essential in COVID-19 and digestive system cancers. Furthermore, six related transcription factors (E2F1, E2F3, E2F4, MYC, TP53, YBX1) were involved in hub gene regulation. Via in vitro experiments, , , and expression was verified in 25 colorectal cancer tissue pairs. Our study revealed the key biomarks and common pathogenesis of digestive system cancers and COVID-19. These may provide new ideas for further mechanistic research.

摘要

消化系统癌症和新型冠状病毒肺炎（COVID-19）对消化系统有显著影响，但COVID-19与消化系统癌症之间的相互作用机制尚未完全阐明。我们从基因表达综合数据库（GEO）下载了COVID-19和七种消化系统癌症（口腔、食管、胃、结肠、肝细胞、胆管、胰腺）的基因表达数据，并鉴定了核心差异表达基因。对核心基因进行了多次验证、诊断效能、预后分析、功能富集及相关转录因子研究。我们鉴定出23个共同差异表达基因用于后续分析。CytoHubba鉴定出9个核心基因（……）。肿瘤基因组图谱（TCGA）和GEO数据验证了核心基因的表达以及出色的诊断和预后能力。功能分析表明，细胞分裂和细胞周期过程在COVID-19和消化系统癌症中至关重要。此外，六个相关转录因子（E2F1、E2F3、E2F4、MYC、TP53、YBX1）参与核心基因调控。通过体外实验，在25对结直肠癌组织中验证了……的表达。我们的研究揭示了消化系统癌症和COVID-19的关键生物标志物及共同发病机制。这些可能为进一步的机制研究提供新思路。