Yang Hua, Cai Yan, Zhuo Zhihang, Yang Wei, Yang Chunping, Zhang Jin, Yang Yang, Wang Baoxin, Guan Fengrong
Key Laboratory of Ecological Forestry Engineering of Sichuan Province, College of Forestry, Sichuan Agricultural University, Chengdu, Sichuan, China.
Chengdu Academy of Agriculture and Forestry Sciences, Chengdu, Sichuan, China.
PLoS One. 2018 Feb 23;13(2):e0192730. doi: 10.1371/journal.pone.0192730. eCollection 2018.
The white-striped longhorn beetle Batocera horsfieldi (Coleoptera: Cerambycidae) is a polyphagous wood-boring pest that causes substantial damage to the lumber industry. Moreover olfactory proteins are crucial components to function in related processes, but the B. horsfieldi genome is not readily available for olfactory proteins analysis. In the present study, developmental transcriptomes of larvae from the first instar to the prepupal stage, pupae, and adults (females and males) from emergence to mating were built by RNA sequencing to establish a genetic background that may help understand olfactory genes. Approximately 199 million clean reads were obtained and assembled into 171,664 transcripts, which were classified into 23,380, 26,511, 22,393, 30,270, and 87, 732 unigenes for larvae, pupae, females, males, and combined datasets, respectively. The unigenes were annotated against NCBI's non-redundant nucleotide and protein sequences, Swiss-Prot, Gene Ontology (GO), Pfam, Clusters of Eukaryotic Orthologous Groups (KOG), and KEGG Orthology (KO) databases. A total of 43,197 unigenes were annotated into 55 sub-categories under the three main GO categories; 25,237 unigenes were classified into 26 functional KOG categories, and 25,814 unigenes were classified into five functional KEGG Pathway categories. RSEM software identified 2,983, 3,097, 870, 2,437, 5,161, and 2,882 genes that were differentially expressed between larvae and males, larvae and pupae, larvae and females, males and females, males and pupae, and females and pupae, respectively. Among them, genes encoding seven candidate odorant binding proteins (OBPs) and three chemosensory proteins (CSPs) were identified. RT-PCR and RT-qPCR analyses showed that BhorOBP3, BhorCSP2, and BhorOBPC1/C3/C4 were highly expressed in the antenna of males, indicating these genes may may play key roles in foraging and host-orientation in B. horsfieldi. Our results provide valuable molecular information about the olfactory system in B. horsfieldi and will help guide future functional studies on olfactory genes.
白带长角天牛（Batocera horsfieldi，鞘翅目：天牛科）是一种多食性蛀木害虫，对木材行业造成了巨大损失。此外，嗅觉蛋白是相关过程中发挥功能的关键成分，但白带长角天牛的基因组不易用于嗅觉蛋白分析。在本研究中，通过RNA测序构建了从一龄幼虫到预蛹期幼虫、蛹以及羽化至交配期成虫（雌性和雄性）的发育转录组，以建立有助于理解嗅觉基因的遗传背景。共获得约1.99亿条clean reads，并组装成171,664条转录本，分别为幼虫、蛹、雌性、雄性和合并数据集分类得到23,380、26,511、22,393、30,270和87,732个单基因。这些单基因与NCBI的非冗余核苷酸和蛋白质序列、Swiss-Prot、基因本体论（GO）、Pfam、真核直系同源簇（KOG）和KEGG直系同源（KO）数据库进行比对注释。共有43,197个单基因被注释到三个主要GO类别下的55个子类别中；25,237个单基因被分类到26个功能KOG类别中，25,814个单基因被分类到五个功能KEGG通路类别中。RSEM软件鉴定出分别在幼虫与雄性、幼虫与蛹、幼虫与雌性、雄性与雌性、雄性与蛹以及雌性与蛹之间差异表达的2,983、3,097、870、2,437、5,
