Corrêa-Junior Dario, Bastos de Andrade Iara, Alves Vinicius, Avellar-Moura Igor, Brito de Souza Rabello Vanessa, Valdez Alessandro Fernandes, Nimrichter Leonardo, Zancopé-Oliveira Rosely Maria, Ribeiro de Sousa Araújo Glauber, Almeida-Paes Rodrigo, Frases Susana
Laboratório de Biofísica de Fungos, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-853, Brazil.
Laboratório de Micologia, Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro 21040-900, Brazil.
J Fungi (Basel). 2023 Jun 25;9(7):701. doi: 10.3390/jof9070701.
Sporotrichosis is a fungal infection caused by species, with as a prevalent pathogen in Latin America. Despite its clinical importance, the virulence factors of and their impact on the pathogenesis of sporotrichosis are still poorly understood. This study evaluated the morphostructural plasticity of , a fungus that causes sporotrichosis. Three cell surface characteristics, namely cell surface hydrophobicity, Zeta potential, and conductance, were assessed. Biofilm formation was also analyzed, with measurements taken for biomass, extracellular matrix, and metabolic activity. In addition, other potential and poorly studied characteristics correlated with virulence such as lipid bodies, chitin, and cell size were evaluated. The results revealed that the major phenotsypic features associated with fungal virulence in the studied strains were chitin, lipid bodies, and conductance. The dendrogram clustered the strains based on their overall similarity in the production of these factors. Correlation analyses showed that hydrophobicity was strongly linked to the production of biomass and extracellular matrix, while there was a weaker association between Zeta potential and size, and lipid bodies and chitin. This study provides valuable insights into the virulence factors of and their potential role in the pathogenesis of sporotrichosis.
孢子丝菌病是由某些菌种引起的真菌感染，在拉丁美洲，申克孢子丝菌是一种常见的病原体。尽管其具有临床重要性，但申克孢子丝菌的毒力因子及其对孢子丝菌病发病机制的影响仍知之甚少。本研究评估了引起孢子丝菌病的申克孢子丝菌的形态结构可塑性。评估了三个细胞表面特征，即细胞表面疏水性、Zeta电位和电导率。还分析了生物膜形成情况，对生物量、细胞外基质和代谢活性进行了测量。此外，还评估了其他与毒力相关但研究较少的潜在特征，如脂质体、几丁质和细胞大小。结果显示，在所研究的申克孢子丝菌菌株中，与真菌毒力相关的主要表型特征是几丁质、脂质体和电导率。聚类图根据这些因素产生的总体相似性对菌株进行了聚类。相关性分析表明，疏水性与生物量和细胞外基质的产生密切相关，而Zeta电位与大小、脂质体与几丁质之间的关联较弱。本研究为申克孢子丝菌的毒力因子及其在孢子丝菌病发病机制中的潜在作用提供了有价值的见解。
