Permyakova Elizaveta S, Manakhov Anton, Kiryukhantsev-Korneev Philipp V, Konopatsky Anton S, Makarets Yulia A, Kotyakova Kristina Yu, Filippovich Svetlana Yu, Ignatov Sergey G, Solovieva Anastasiya O, Shtansky Dmitry V
National University of Science and Technology "MISIS", Moscow 119049, Russia.
Research Institute of Clinical and Experimental Lymphology-Branch of the ICG SB RAS, 2 Timakova st., Novosibirsk 630060, Russia.
J Funct Biomater. 2023 Jun 25;14(7):336. doi: 10.3390/jfb14070336.
The objective of this research was to develop an environment-friendly and scalable method for the production of self-sanitizing electrospun nanofibers. This was achieved by immobilizing silver nanoparticles (Ag NPs) onto plasma-treated surfaces of biodegradable polycaprolactone (PCL) nanofibers. The plasma deposited polymer layer containing carboxyl groups played a critical role in providing a uniform distribution of Ag NPs on the nanofiber surface. Ag ions were absorbed by electrostatic interaction and then reduced under the action of UV-light. The concentration and release of Ag ions were analyzed using the EDXS/XPS and ICP AES methods, respectively. Although high levels of Ag ions were detected after 3 h of immersion in water, the material retained a sufficient amount of silver nanoparticles on the surface (~2.3 vs. 3.5 at.% as determined by XPS), and the release rate subsequently decreased over the next 69 h. The antipathogenic properties of PCL-Ag were tested against gram-negative and gram-positive bacteria, fungi, and biofilm formation. The results showed that the PCL-Ag nanofibers exhibit significant antimicrobial activity against a wide range of microorganisms, including those that cause human infections. The incorporation of Ag NPs into PCL nanofibers resulted in a self-sanitizing material that can be used in variety of applications, including wound dressings, water treatment, and air filtration. The development of a simple, scalable, and environmentally friendly method for the fabrication of these nanofibers is essential to ensure their widespread use in various industries. The ability to control the concentration and release rate of Ag ions in the PCL nanofibers will be critical to optimize their efficacy while minimizing their potential toxicity to human cells and the environment.
本研究的目的是开发一种环境友好且可扩展的方法来生产自消毒电纺纳米纤维。这是通过将银纳米颗粒（Ag NPs）固定在可生物降解的聚己内酯（PCL）纳米纤维经等离子体处理的表面上来实现的。含有羧基的等离子体沉积聚合物层在使Ag NPs在纳米纤维表面均匀分布方面起着关键作用。Ag离子通过静电相互作用被吸收，然后在紫外光的作用下被还原。分别使用能量过滤X射线能谱/ X射线光电子能谱（EDXS/XPS）和电感耦合等离子体原子发射光谱法（ICP AES）分析Ag离子的浓度和释放情况。尽管在水中浸泡3小时后检测到高水平的Ag离子，但该材料在表面保留了足够数量的银纳米颗粒（通过XPS测定为~2.3原子％对3.5原子％），并且在接下来的69小时内释放速率随后下降。测试了PCL-Ag对革兰氏阴性菌和革兰氏阳性菌、真菌以及生物膜形成的抗病原特性。结果表明，PCL-Ag纳米纤维对多种微生物具有显著的抗菌活性，包括那些引起人类感染的微生物。将Ag NPs掺入PCL纳米纤维中产生了一种自消毒材料，可用于多种应用，包括伤口敷料、水处理和空气过滤。开发一种简单、可扩展且环境友好的方法来制造这些纳米纤维对于确保它们在各个行业中的广泛应用至关重要。控制PCL纳米纤维中Ag离子的浓度和释放速率的能力对于优化其功效同时最小化其对人类细胞和环境的潜在毒性至关重要。
