Puccetti Matteo, Donnadio Anna, Ricci Maurizio, Latterini Loredana, Quaglia Giulia, Pietrella Donatella, Di Michele Alessandro, Ambrogi Valeria
Dipartimento di Scienze Farmaceutiche, Università degli Studi di Perugia, Via del Liceo 1, 06123 Perugia, Italy.
Nano4Light Lab, Dipartimento di Chimica, Biologia e Biotecnologie, Università degli Studi di Perugia, Via Elce di Sotto, 8, 06123 Perugia, Italy.
J Funct Biomater. 2023 Feb 1;14(2):84. doi: 10.3390/jfb14020084.
Recently, silver-based nanoparticles have been proposed as components of wound dressings due to their antimicrobial activity. Unfortunately, they are cytotoxic for keratinocytes and fibroblasts, and this limits their use. Less consideration has been given to the use of AgCl nanoparticles in wound dressings. In this paper, a sustainable preparation of alginate AgCl nanoparticles composite films by simultaneous alginate gelation and AgCl nanoparticle formation in the presence of CaCl solution is proposed with the aim of obtaining films with antimicrobial and antibiofilm activities and low cytotoxicity. First, AgNO alginate films were prepared, and then, gelation and nanoparticle formation were induced by film immersion in CaCl solution. Films characterization revealed the presence of both AgCl and metallic silver nanoparticles, which resulted as quite homogeneously distributed, and good hydration properties. Finally, films were tested for their antimicrobial and antibiofilm activities against (ATCC 12228), (ATCC 29213), (ATCC 15692), and the yeast . Composite films showed antibacterial and antibiofilm activities against the tested bacteria and resulted as less active towards . Film cytotoxicity was investigated towards human dermis fibroblasts (HuDe) and human skin keratinocytes (NCTC2544). Composite films showed low cytotoxicity, especially towards fibroblasts. Thus, the proposed sustainable approach allows to obtain composite films of Ag/AgCl alginate nanoparticles capable of preventing the onset of infections without showing high cytotoxicity for tissue cells.
最近，基于银的纳米颗粒因其抗菌活性被提议作为伤口敷料的成分。不幸的是，它们对角质形成细胞和成纤维细胞具有细胞毒性，这限制了它们的使用。人们较少考虑将氯化银纳米颗粒用于伤口敷料。本文提出了一种在氯化钙溶液存在下通过海藻酸盐同时凝胶化和形成氯化银纳米颗粒来可持续制备海藻酸银氯化银纳米颗粒复合膜的方法，目的是获得具有抗菌和抗生物膜活性且细胞毒性低的膜。首先，制备硝酸银海藻酸盐膜，然后通过将膜浸入氯化钙溶液中诱导凝胶化和纳米颗粒形成。膜的表征显示同时存在氯化银和金属银纳米颗粒，它们分布相当均匀，并且具有良好的水合性能。最后，测试了膜对金黄色葡萄球菌（ATCC 12228）、大肠杆菌（ATCC 29213）、白色念珠菌（ATCC 15692）和酵母菌的抗菌和抗生物膜活性。复合膜对测试细菌显示出抗菌和抗生物膜活性，对白色念珠菌的活性较低。研究了复合膜对人真皮成纤维细胞（HuDe）和人皮肤角质形成细胞（NCTC2544）的细胞毒性。复合膜显示出低细胞毒性，尤其是对成纤维细胞。因此，所提出的可持续方法能够获得银/氯化银海藻酸盐纳米颗粒复合膜，该复合膜能够预防感染发生，且对组织细胞不显示高细胞毒性。
