College of Life Science, Liaoning Normal University, Dalian 116081, China.
Lamprey Research Center, Liaoning Normal University, Dalian 116081, China.
Mar Drugs. 2023 Jun 29;21(7):389. doi: 10.3390/md21070389.
Various proteins with antibacterial, anticoagulant, and anti-inflammatory properties have been identified in the buccal glands of jawless blood-sucking vertebrate lampreys. However, studies on endogenous peptides in the buccal gland of lampreys are limited. In this study, 4528 endogenous peptides were identified from 1224 precursor proteins using peptidomics and screened for bioactivity in the buccal glands of the lamprey, . We synthesized four candidate bioactive peptides (VSLNLPYSVVRGEQFVVQA, DIPVPEVPILE, VVQLPPVVLGTFG, and VPPPPLVLPPASVK), calculated their secondary structures, and validated their bioactivity. The results showed that the peptide VSLNLPYSVVRGEQFVVQA possessed anti-inflammatory activity, which significantly increased the expression of anti-inflammatory factors and decreased the expression of inflammatory factors in THP-1 cells. The peptide VVQLPPVVLGTFG showed antibacterial activity against some gram-positive bacteria. The peptide VSLNLPYSVVRGEQFVQA possessed good ACE inhibitory activity at low concentrations, but no dose-related correlation was observed. Our study revealed that the buccal glands of the jawless vertebrate lamprey are a source of multiple bioactive peptides, which will provide new insights into the blood-sucking mechanism of lamprey.
在无颚吸血脊椎动物七鳃鳗的颊腺中发现了具有抗菌、抗凝和抗炎特性的各种蛋白质。然而，关于七鳃鳗颊腺内源性肽的研究有限。在这项研究中，使用肽组学从 1224 种前体蛋白中鉴定出 4528 种内源性肽，并筛选了七鳃鳗颊腺中的生物活性。我们合成了四个候选生物活性肽（VSLNLPYSVVRGEQFVVQA、DIPVPEVPILE、VVQLPPVVLGTFG 和 VPPPPLVLPPASVK），计算了它们的二级结构，并验证了它们的生物活性。结果表明，肽 VSLNLPYSVVRGEQFVVQA 具有抗炎活性，可显著增加 THP-1 细胞中抗炎因子的表达，降低炎症因子的表达。肽 VVQLPPVVLGTFG 对一些革兰氏阳性菌具有抗菌活性。肽 VSLNLPYSVVRGEQFVQA 在低浓度时具有良好的 ACE 抑制活性，但未观察到剂量相关性。我们的研究表明，无颚脊椎动物七鳃鳗的颊腺是多种生物活性肽的来源，这将为七鳃鳗吸血机制提供新的见解。
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