Centro de Biotecnologia e Química Fina (CBQF)-Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal.
Human Neurobehavioral Laboratory/Research Centre for Human Development (HNL/CEDH), Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal.
Int J Mol Sci. 2022 Feb 23;23(5):2439. doi: 10.3390/ijms23052439.
Food-derived bioactive peptides are of great interest to science and industry due to evolving drivers of food product innovation, including health and wellness. This study aims to draw attention through a critical study on how bioinformatics analysis is employed in the identification of bioactive peptides in the laboratory. An in silico analysis (PeptideRanker, BIOPEP, AHTpin, and mAHTPred) of a list of peptides from goat casein hydrolysate was performed to predict which sequences could potentially be bioactive. To validate the predictions, the in vitro antihypertensive potential of the five peptides with the highest potential was first measured. Then, for three of these, gastrointestinal digestion was simulated in vitro, followed by the analysis of the resulting ACE inhibitory activity as well as antioxidant capacity. We thus observed that the use of new computational biology technologies to predict peptide sequences is an important research tool, but they should not be used alone and complementarity with various in vitro and in vivo assays is essential.
由于食品产品创新的驱动因素不断发展，包括健康和保健，因此食品来源的生物活性肽引起了科学界和工业界的极大兴趣。本研究旨在通过对生物信息学分析在实验室中鉴定生物活性肽的应用进行批判性研究，引起人们的关注。对来自山羊乳清蛋白水解物的肽列表进行了计算机分析（PeptideRanker、BIOPEP、AHTpin 和 mAHTPred），以预测哪些序列可能具有生物活性。为了验证预测结果，首先测量了具有最高潜力的五个肽的体外降压潜力。然后，对其中的三个进行了体外胃肠道消化模拟，随后分析了所得 ACE 抑制活性和抗氧化能力。因此，我们观察到，使用新的计算生物学技术来预测肽序列是一种重要的研究工具，但不应单独使用，并且与各种体外和体内测定互补是必不可少的。
