Jiang Liqun, Yu Siteng, Chen Huiying, Pei Haiyan
Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, China; School of Environmental Science and Engineering, Shandong University, Qingdao 266237, China; Shandong Provincial Engineering Center on Environmental Science and Technology, Jinan 250061, China.
School of Environmental Science and Engineering, Shandong University, Qingdao 266237, China.
Bioresour Technol. 2023 Jun;378:129009. doi: 10.1016/j.biortech.2023.129009. Epub 2023 Apr 1.
To find out optimum and cost-efficient strategy for phycocyanin production, the effect of light source and temperature on Spirulina subsalsa growth were studied in chemically defined freshwater medium and seawater supplied with wastewater from glutamic acid fermentation tank. Maximum growth rate and the highest phycocyanin content were obtained by 35 °C and green light, respectively. A two-stage cultivation strategy was proposed and applied, which combines biomass accumulation at 35 °C and phycocyanin synthesis simulated under green light. As a result, phycocyanin production reached 70 mg/L/d and 11 mg/L/d from freshwater and seawater medium, respectively. With all tested conditions, a strong correlation between biomass and phycocyanin/chlorophyll ratio, rather than phycocyanin, revealed the dependence of Spirulina subsalsa growth on coordinating regulation of photosynthetic pigments. The relationship between growth and phycocyanin production under various light and temperature can be a good basis for improving phycocyanin production from Spirulina subsalsa with or without freshwater consumption.
为了找出生产藻蓝蛋白的最佳且经济高效的策略，在化学限定的淡水培养基以及添加了谷氨酸发酵罐废水的海水中，研究了光源和温度对盐生螺旋藻生长的影响。分别在35℃和绿光条件下获得了最大生长速率和最高藻蓝蛋白含量。提出并应用了两阶段培养策略，该策略将35℃下的生物量积累与绿光模拟下的藻蓝蛋白合成相结合。结果，淡水和海水培养基中的藻蓝蛋白产量分别达到70mg/L/d和11mg/L/d。在所有测试条件下，生物量与藻蓝蛋白/叶绿素比率之间存在很强的相关性，而非与藻蓝蛋白的相关性，这揭示了盐生螺旋藻生长对光合色素协调调节的依赖性。各种光照和温度条件下生长与藻蓝蛋白生产之间的关系，可为提高盐生螺旋藻藻蓝蛋白产量（无论是否消耗淡水）提供良好依据。
