School of Life Sciences and Medicine, Shandong University of Technology, Zibo 255000, Shandong Province, PR China.
College of Life Sciences, Shandong Agricultural University, Taian 271018, Shandong Province, PR China.
Int J Syst Evol Microbiol. 2023 Jul;73(7). doi: 10.1099/ijsem.0.005992.
A Gram-stain-negative, strictly aerobic, non-motile, catalase- and oxidase-positive, pink and rod-shaped strain, designated RY-2, was isolated from sediment of Fuyang River located in Wuqiang County, Hengshui City, Hebei Province, PR China. The strain grew at 25-45 °C (optimum, 37 °C), pH 7.0-8.0 (optimum, pH 7.0) and in the presence of 0-1.5 % (w/v) NaCl (optimum, 1 %). From the phylogenetic analysis of the 16S rRNA gene sequence, strain RY-2 was affiliated to the genus , and had the highest 16S rRNA gene sequence similarity to JCM 17389 (98.3 %) and the similarity values between strain RY-2 and other type strains was all below 89.3 %. The genome size of strain RY-2 was 4.75 Mb and the DNA G+C content was 46.6 %. Values of digital DNA-DNA hybridization and average nucleotide identity between strain RY-2 and the reference strain were 63.2 and 95.5 %, respectively. The major fatty acids (≥5.0 %) were iso-C (37.9 %), summed feature 9 (8.4 %, iso-C 9 and/or C10-methyl), anteiso-C (8.2 %), iso-C 3-OH (7.6 %) and summed feature 4 (5.2 %, iso-C I and/or anteiso-C B) and its sole menaquinone was MK-7. The polar lipids consisted of phosphatidylethanolamine, an unknown phosphoglycolipid, an unidentified phospholipid, two unidentified aminolipids, three unidentified glycolipids and nine unidentified lipids. Based on the results of biochemical, physiological, phylogenomic and chemotaxonomic analyses, strain RY-2 is considered to represent a novel species of the genus within the family , for which the name sp. nov. is proposed. The type strain is RY-2 (=GDMCC 1.2781=JCM 35631).
一株革兰氏阴性、严格需氧、不运动、接触酶和氧化酶阳性、粉红色、杆状的菌株，被命名为 RY-2，从位于中国河北省衡水市武强县的滏阳河沉积物中分离得到。该菌株在 25-45°C（最佳温度为 37°C）、pH 值 7.0-8.0（最佳 pH 值为 7.0）和 0-1.5%（w/v）NaCl（最佳为 1%）的条件下生长。从 16S rRNA 基因序列的系统发育分析来看，菌株 RY-2 属于 属，与 JCM 17389 的 16S rRNA 基因序列相似性最高（98.3%），与其他模式菌株的相似性值均低于 89.3%。菌株 RY-2 的基因组大小为 4.75Mb，DNA G+C 含量为 46.6%。菌株 RY-2 与参考菌株之间的数字 DNA-DNA 杂交值和平均核苷酸同一性值分别为 63.2%和 95.5%。主要脂肪酸（≥5.0%）为 iso-C（37.9%）、summed feature 9（8.4%，iso-C 9 和/或 C10-methyl）、anteiso-C（8.2%）、iso-C 3-OH（7.6%）和 summed feature 4（5.2%，iso-C I 和/或 anteiso-C B），其唯一的menaquinone 为 MK-7。极性脂包括磷脂酰乙醇胺、一种未知的磷酸甘油脂、一种未鉴定的磷脂、两种未鉴定的氨基脂、三种未鉴定的糖脂和九种未鉴定的脂类。基于生化、生理、系统发育和化学分类学分析的结果，菌株 RY-2 被认为代表了 属内的一个新种，建议将其命名为 sp. nov.。该模式菌株为 RY-2（=GDMCC 1.2781=JCM 35631）。
