sp. nov., a novel thermotolerant bacterium isolated from river silt.

A yellow, Gram-stain-positive, strictly aerobic, thermotolerant, non-motile and rod-shaped bacterial strain, designated RY-1, was isolated from a silt sample of Fuyang River, Wuqiang County, Hengshui City, Hebei Province, PR China. Cells showed oxidase- and catalase-positive activities. Growth occurred at 20-45 °C (optimum, 37 °C) and pH 6.0-8.0 (optimum, pH 7.0), and in the presence of 0-1.5 % (w/v) NaCl (optimum, 0%). A phylogenetic tree based on 16S rRNA gene sequences revealed that strain RY-1 formed a phylogenetic lineage with members within the family . A comparison of 16S rRNA gene sequences showed that strain RY-1 was most closely related to WS16 (98.6 %), CJ663 (97.7 %) and 3-3 (97.6 %). The genome size of strain RY-1 was 4.71 Mb, and the DNA G+C content was 44.3  %. The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain RY-1 and reference strains were all lower than the threshold values for species delineation. Strain RY-1 contained menaquinone-7 and iso-C, iso-C 3-OH and iso-CG as the sole respiratory isoprenoid quinone and major cellular fatty acids (≥5 %), respectively. The major polar lipids consisted of phosphatidylethanolamine, three unidentified aminolipids and four unidentified lipids. According to the results of phenotypic, phylogenetic and chemotaxonomic characteristics, strain RY-1 represents a novel species of the genus , for which the name p. nov. is proposed. The type strain is RY-1 (=GDMCC 1.2775=JCM 34870).