School of Life Sciences and Medicine, Shandong University of Technology, Zibo, 255000, Shandong Province, PR China.
School of Resources and Environmental Engineering, Shandong University of Technology, Zibo, 255000, Shandong Province, PR China.
Int J Syst Evol Microbiol. 2023 Feb;73(1). doi: 10.1099/ijsem.0.005619.
A yellow, Gram-stain-positive, strictly aerobic, thermotolerant, non-motile and rod-shaped bacterial strain, designated RY-1, was isolated from a silt sample of Fuyang River, Wuqiang County, Hengshui City, Hebei Province, PR China. Cells showed oxidase- and catalase-positive activities. Growth occurred at 20-45 °C (optimum, 37 °C) and pH 6.0-8.0 (optimum, pH 7.0), and in the presence of 0-1.5 % (w/v) NaCl (optimum, 0%). A phylogenetic tree based on 16S rRNA gene sequences revealed that strain RY-1 formed a phylogenetic lineage with members within the family . A comparison of 16S rRNA gene sequences showed that strain RY-1 was most closely related to WS16 (98.6 %), CJ663 (97.7 %) and 3-3 (97.6 %). The genome size of strain RY-1 was 4.71 Mb, and the DNA G+C content was 44.3 %. The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain RY-1 and reference strains were all lower than the threshold values for species delineation. Strain RY-1 contained menaquinone-7 and iso-C, iso-C 3-OH and iso-CG as the sole respiratory isoprenoid quinone and major cellular fatty acids (≥5 %), respectively. The major polar lipids consisted of phosphatidylethanolamine, three unidentified aminolipids and four unidentified lipids. According to the results of phenotypic, phylogenetic and chemotaxonomic characteristics, strain RY-1 represents a novel species of the genus , for which the name p. nov. is proposed. The type strain is RY-1 (=GDMCC 1.2775=JCM 34870).
一株黄色、革兰氏阳性、严格需氧、耐热、非运动、杆状的细菌菌株，被命名为 RY-1，从中国河北省衡水市武强县滏阳河的淤泥样本中分离得到。细胞表现出氧化酶和过氧化氢酶阳性活性。生长发生在 20-45°C（最佳温度为 37°C）和 pH 值 6.0-8.0（最佳 pH 值为 7.0），并在 0-1.5%（w/v）NaCl（最佳为 0%）存在的情况下。基于 16S rRNA 基因序列的系统发育树显示，菌株 RY-1 与. 科的成员形成了一个系统发育分支。16S rRNA 基因序列比较表明，菌株 RY-1 与 WS16（98.6%）、CJ663（97.7%）和 3-3（97.6%）最为密切相关。菌株 RY-1 的基因组大小为 4.71 Mb，DNA G+C 含量为 44.3%。菌株 RY-1 与参考菌株之间的平均核苷酸同一性、数字 DNA-DNA 杂交和平均氨基酸同一性值均低于物种划分的阈值。菌株 RY-1 含有menaquinone-7 和 iso-C、iso-C 3-OH 和 iso-CG 作为唯一的呼吸异戊二烯醌和主要的细胞脂肪酸（≥5%）。主要极性脂类由磷脂酰乙醇胺、三种未鉴定的胺脂和四种未鉴定的脂类组成。根据表型、系统发育和化学分类学特征，菌株 RY-1 代表. 属的一个新种，建议将其命名为 p. nov.。模式菌株为 RY-1（=GDMCC 1.2775=JCM 34870）。
