Department of Anatomic Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
Department of Pulmonology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
Asian Pac J Cancer Prev. 2023 Jul 1;24(7):2329-2335. doi: 10.31557/APJCP.2023.24.7.2329.
The objective was to evaluate the expression of melanoma antigen (MAGE) A from A1 to 10 (A1-10) and the individual MAGE A family in the peripheral lung tumors and to analyze its association with histopathological findings.
A cross-sectional study was conducted on 67 samples of peripheral lung tumor obtained by core biopsies from patients with clinical diagnoses such as lung and mediastinal tumors. The specimens were divided into two, one to perform histopathological diagnosis and the last for mRNA MAGE A examination. A Nested polymerase chain reaction (PCR) was performed using universal primer, MF10/MR10 and MF10/MR12. The collected data were analyzed by appropriate statistical techniques.
The histopathological finding showed 41 (61.2 %) of specimens as malignant cells and 26 (38.8 %) of specimens as non-malignant cells. MAGE A1-10 was expressed at 47 (70.1 %) and MAGE A1-6 was expressed at 25 (37.3 %) of specimens. In a malignant cell, MAGE A1-10 and MAGE A1-6 were expressed at 33 (80.5 %) and 19 (46.3 %), respectively. In non-malignant cells, MAGE A1-10 and MAGE A1-6 were expressed at 14 (53.9 %) and 6 (23.1 %,) respectively. The MAGE A1-10 and MAGE A8 expressions were significantly associated with histopathological findings of malignant or non-malignant cells. The sensitivity, specificity, and diagnostic accuracy of MAGE A1-10 were 80.5 %, 46.2 %, and 67.2 %, respectively; while for MAGE A8 were 41.5 %, 88.5 %, and 59.7 %, respectively.
The MAGE A1-10 expression was the most commonly detected and associated with the histopathological finding. Moreover, it was more sensitive and specific and had higher diagnostic accuracy than others. Therefore, the MAGE A1-10 assay may improve the accuracy of the diagnosis of malignancy in peripheral lung tumors.
评估黑色素瘤相关抗原（MAGE）A1 至 10（A1-10）和 MAGE A 家族个体在肺外周肿瘤中的表达，并分析其与组织病理学发现的关系。
对 67 例经临床诊断为肺和纵隔肿瘤的患者进行经皮肺外周肿瘤核心活检获得的样本进行了一项横断面研究。将标本分为两部分，一部分用于进行组织病理学诊断，另一部分用于进行 mRNA MAGE A 检查。采用通用引物 MF10/MR10 和 MF10/MR12 进行巢式聚合酶链反应（PCR）。采用适当的统计技术分析收集的数据。
组织病理学发现 41 例（61.2%）标本为恶性细胞，26 例（38.8%）标本为非恶性细胞。47 例（70.1%）标本表达 MAGE A1-10，25 例（37.3%）标本表达 MAGE A1-6。在恶性细胞中，MAGE A1-10 和 MAGE A1-6 的表达率分别为 33 例（80.5%）和 19 例（46.3%）。在非恶性细胞中，MAGE A1-10 和 MAGE A1-6 的表达率分别为 14 例（53.9%）和 6 例（23.1%）。MAGE A1-10 和 MAGE A8 的表达与恶性或非恶性细胞的组织病理学发现显著相关。MAGE A1-10 的灵敏度、特异性和诊断准确性分别为 80.5%、46.2%和 67.2%；而 MAGE A8 分别为 41.5%、88.5%和 59.7%。
MAGE A1-10 的表达最常见且与组织病理学发现相关。此外，它比其他基因更敏感、更特异，诊断准确性更高。因此，MAGE A1-10 检测可能会提高肺外周肿瘤恶性诊断的准确性。
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