脐带间充质干细胞来源的外泌体对口腔鳞状细胞癌的作用（体外研究）。
The Effects of Umbilical Cord Mesenchymal Stem Cells -Derived Exosomes in Oral Squamous Cell Carcinoma (In vitro Study).
机构信息
Lecturer of Oral Medicine, Diagnosis and Periodontology, Faculty of Dentistry, October 6 University, Cairo, Egypt.
Lecturer of Oral and Maxillofacial Pathology, Faculty of Dentistry, October 6 University, Cairo, Egypt.
出版信息
Asian Pac J Cancer Prev. 2023 Jul 1;24(7):2531-2542. doi: 10.31557/APJCP.2023.24.7.2531.
OBJECTIVE
Mesenchymal stem cells (MSCs) derived exosomes offers several advantages as a cell-free therapeutic agents. In this study, Umbilical cord mesenchymal stem cells exosomes (UC-MSCs-exos) effects on oral squamous cell carcinoma (OSCC) cell line was evaluated.
METHODS
UC-MSCs-exos were isolated and co-cultured with OSCC cells and their impact on OSCC was explored by various tests. Comet assay and western blot for cleaved caspase-3 and immunocytochemistry for caspase-8 were used for apoptosis assessment. HO-1 and Nrf2 were used to determine antioxidant levels. Tumor necrosis factor-α and interleukin-6 were assessed as inflammatory biomarkers. HOX transcript antisense intergenic long noncoding RNA (HOTAIR) expression was also evaluated.
RESULTS
In a dose-dependent manner, UC-MSCs-exos reduced the levels of pro-inflammatory cytokines (IL-6 and TNF-α) and induced apoptosis of OSCC in vitro. Meanwhile, we found that UC-MSCs-exos downregulate HOTAIR.
CONCLUSION
UC-MSCs-exos conferred a suppressive role on OSCC in vitro, highlighting a promising therapeutic role. However, the exact potentially involved molecules and molecular mechanisms need to be investigated in further studies.
目的
间充质干细胞（MSCs）衍生的外泌体作为无细胞治疗剂具有多种优势。在这项研究中，评估了脐带间充质干细胞外泌体（UC-MSCs-exos）对口腔鳞状细胞癌（OSCC）细胞系的影响。
方法
分离 UC-MSCs-exos 并与 OSCC 细胞共培养，通过各种测试探索其对 OSCC 的影响。彗星试验和裂解 caspase-3 的 Western blot 以及 caspase-8 的免疫细胞化学用于评估细胞凋亡。HO-1 和 Nrf2 用于确定抗氧化水平。肿瘤坏死因子-α和白细胞介素-6 作为炎症生物标志物进行评估。还评估了 HOX 转录反义基因间长非编码 RNA（HOTAIR）的表达。
结果
UC-MSCs-exos 以剂量依赖的方式降低了促炎细胞因子（IL-6 和 TNF-α）的水平，并诱导了 OSCC 的体外凋亡。同时，我们发现 UC-MSCs-exos 下调了 HOTAIR。
结论
UC-MSCs-exos 在体外对 OSCC 具有抑制作用，突出了其有希望的治疗作用。然而，确切的潜在涉及分子和分子机制需要在进一步的研究中进行调查。
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