Andonova Maria, Dzhelebov Petko, Trifonova Krastina, Yonkova Penka, Kostadinov Nikola, Nancheva Krasimira, Ivanov Veselin, Gospodinova Krasimira, Nizamov Nikola, Tsachev Ilia, Chernev Chavdar
Department of General and Clinical Pathology, Faculty of Veterinary Medicine, Trakia University, Stara Zagora 6000, Bulgaria.
Department of Veterinary Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, Trakia University, Stara Zagora 6000, Bulgaria.
Vet Sci. 2023 Jul 2;10(7):431. doi: 10.3390/vetsci10070431.
Science is still searching for readily available, cost-effective biomarkers to assess metabolic disorders occurring before the onset and during the development of type-2 diabetes (T2DM). The aim of the present study was to induce T2DM in rats through a high-fat diet, followed by a single administration of low dose streptozotocin (STZ), and make an assessment of the development of the disease. The rats were divided into two groups-experimental and control-and were monitored for a period of 10 days. Changes in anthropometric parameters, glucose, insulin, lipids, uric acid, advanced oxidation protein products (AOPP), as well as the histological changes in the liver and pancreas, were recorded. To assess insulin resistance, we used the Homeostasis Model Assessment of Insulin Resistance (HOMA-IR) and beta cell function (HOMA-β) and visceral obesity-adiposity index (AI). The data demonstrate that the increasing values of glucose, HOMA-IR, AI, total cholesterol, triacylglycerols, low- and very-low-density lipoproteins are important markers of the pre-diabetic state. The stable hyperglycemia and increased levels of TC, TG, VLDL, LDL, uric acid and AOPP in experimental rats strongly suggest the development of T2DM. HOMA-IR, HOMA-β, AI, and uric acid are reliable criteria for T2DM in rats.
科学仍在寻找易于获得且具有成本效益的生物标志物，以评估2型糖尿病（T2DM）发病前及发病过程中出现的代谢紊乱。本研究的目的是通过高脂饮食诱导大鼠患T2DM，随后单次给予低剂量链脲佐菌素（STZ），并对疾病的发展进行评估。将大鼠分为两组——实验组和对照组——并监测10天。记录人体测量参数、血糖、胰岛素、血脂、尿酸、晚期氧化蛋白产物（AOPP）的变化，以及肝脏和胰腺的组织学变化。为了评估胰岛素抵抗，我们使用了胰岛素抵抗稳态模型评估（HOMA-IR）、β细胞功能（HOMA-β）和内脏肥胖-肥胖指数（AI）。数据表明，血糖、HOMA-IR、AI、总胆固醇、三酰甘油、低密度脂蛋白和极低密度脂蛋白值的升高是糖尿病前期状态的重要标志物。实验组大鼠持续高血糖以及TC、TG、VLDL、LDL、尿酸和AOPP水平升高，强烈提示T2DM的发展。HOMA-IR、HOMA-β、AI和尿酸是大鼠T2DM的可靠标准。
