Department of Chemical Engineering, Vali-e-Asr University of Rafsanjan, Rafsanjan, Iran; Molecular Medicine Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
Department of Chemical Engineering, Vali-e-Asr University of Rafsanjan, Rafsanjan, Iran.
J Mech Behav Biomed Mater. 2023 Sep;145:106044. doi: 10.1016/j.jmbbm.2023.106044. Epub 2023 Jul 24.
Crosslinked chitosan (CS) is one of the most useable hydrogels in biomedicine and tissue engineering. Unlike most chitosan crosslinkers that are toxic, such as glutaraldehyde, vanillin is a natural, biocompatible, and antimicrobial alternative. The crosslinking of chitosan and vanillin consists of Schiff base bonds between the amines of chitosan and the aldehydes of vanillin, in addition to hydrogen bonds formed across the network. In most studies, the combination of chitosan and vanillin has been investigated in small sizes (micro/nanoscale and biofilms). In this study, a chitosan-vanillin (CV) hydrogel was studied on a macroscale with a three-dimensional porous structure, and it was compared with chitosan crosslinked with glutaraldehyde (CG) on the same scale. Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (FE-SEM) used to identify the bonds formed and examine the morphology of the hydrogels. The gel content, swelling, porosity, mechanical properties, cell viability (on L929 and mesenchymal cells), and antibacterial activity (against Escherichia coli and Staphylococcus aureus) of the samples were investigated. The results showed that the CV had both gel content and high porosity (>90%), with an interconnected porous network of uniform pore size. The CV hydrogel exhibited good antibacterial activity and cell viability. In terms of mechanical properties, CV has weaker mechanical properties compared to CG in the dry state, while the mechanical properties of CV have more improved in the swollen state compared to CG.
交联壳聚糖（CS）是生物医学和组织工程中最常用的水凝胶之一。与大多数毒性的壳聚糖交联剂（如戊二醛）不同，香草醛是一种天然的、生物相容的和抗菌的替代品。壳聚糖和香草醛的交联包括壳聚糖中的氨基与香草醛中的醛基之间的希夫碱键，以及在网络中形成的氢键。在大多数研究中，壳聚糖和香草醛的组合都在较小尺寸（微/纳米级和生物膜）下进行了研究。在这项研究中，研究了具有三维多孔结构的壳聚糖-香草醛（CV）水凝胶，并将其与在相同规模下用戊二醛交联的壳聚糖（CG）进行了比较。傅里叶变换红外光谱（FTIR）和扫描电子显微镜（FE-SEM）用于鉴定形成的键，并检查水凝胶的形态。研究了样品的凝胶含量、溶胀、孔隙率、机械性能、细胞活力（在 L929 和间充质细胞上）和抗菌活性（对大肠杆菌和金黄色葡萄球菌）。结果表明，CV 具有较高的凝胶含量和高孔隙率（>90%），具有均匀孔径的互连多孔网络。CV 水凝胶具有良好的抗菌活性和细胞活力。在机械性能方面，CV 在干燥状态下的机械性能弱于 CG，而在溶胀状态下 CV 的机械性能比 CG 有了更多的改善。
